改良的从肝硬化到产生肝癌的大鼠模型  

A modified approach to establishing a rat model of liver cancer with concurrent cirrhosis

作　　者：周陈杰[1] 宫绪萌[1] 蔡理全[1] 汪艳[1] 高毅[1] 

机构地区：[1]南方医科大学珠江医院肝胆二科,广东广州510282

出　　处：《南方医科大学学报》2014年第4期497-499,共3页Journal of Southern Medical University

基　　金：国家高技术研究发展"863"计划(2012AA020505)~~

摘　　要：目的建立改良的从肝硬化到产生肝癌的大鼠模型，为研究肝癌发病特点和药物干预提供较理想动物模型。方法50只雄性Wistar大鼠（100～120g）随机分为模型组30只、对照组20只。模型组以二乙基亚硝胺（DEN）腹腔注射，50mg/kg体质量，每周2次，连续4周后改为每周1次腹腔注射DEN，50mg/kg体质量，至14周停止；对照组以生理盐水腹腔注射，0．1m瞅，每周2次，连续4周后改为每周1次腹腔注射生理盐水，0．1ml/次，至14周停止。结果诱癌14周以后成功诱导出肝硬化伴发的原发性肝癌，14周时成瘤隋况为3/5，18周时成癌情况为3/3，成癌率为75％，总体死亡率33％（10/30）。结论本模型能精确控制药物给予量，具有操作简便、成癌率高、成癌周期短、死亡率较低等特点，成癌特点与人类类似，是一种较为理想的动物肝癌研究模型。Objective To establish a modified rat model of liver cancer with concurrent cirrhosis for the study of carcinogenesis characteristics and drug intervention of liver cancer. Methods Fifty male Wistar rats weighing 100-120 g were randomly divided into normal control group （20 rats） and model group （30 rats）. In the model group, the rats were subjected to intraperitoneal injection of 50 mg/kg DEN N-diethylnitrosamine （DEN） twice a week for 4 consecutive weeks, followed then by weekly Injections for another 10 weeks. The control rats received injections of 0.1 ml saline in the same manner. At 2, 4, 8, 12, 14, and 18 weeks, 3 rats from each group were sacrificed for assessing tumor formation and liver cirrhosis. Results Liver cancer with concurrent cirrhosis was induced successfully after 14 weeks of DEN injections. At the 14th week, 3 out of the 5 rats were found to have cirrhosis and LC, and at the 18th week all the 3 rats examined had cirrhosis and liver cancer. The total carcinogenesis rate in the rats was 75% at 18 weeks with an overall mortality of 33%. Conclusion This approach to establishing rat models of liver cancer with concurrent cirrhosis requires simple operation, shortens the time of carcinogenesis, and ensures a high success rate of carcinogenesis and a low mortality rate. The carcinogenesis characteristics in this model are similar to those in human.

关 键 词：肝癌 肝硬化 二乙基亚硝胺 大鼠 动物模型 

分 类 号：R735.7[医药卫生—肿瘤]