亚低温对创伤性脑损伤大鼠体感诱发电位和定量脑电图的影响  

Changes of somatosensory evoked potentials and quantitative electroencephalogram in response to mild hypothermia following traumatic brain injury in rats

作　　者：武俏丽[1] 韩璐[2] 蔡英[1] 王辰[1] 莫丽冬[1] 张雪青[2] 黄慧玲[1] 

机构地区：[1]天津市环湖医院,天津市神经外科研究所,天津市脑血管与神经变性重点实验室,300060 [2]天津市环湖医院,天津市神经外科研究所,天津市脑血管与神经电生理科,300060

出　　处：《中华创伤杂志》2014年第4期356-360,共5页Chinese Journal of Trauma

基　　金：天津市应用基础及前沿技术研究计划资助项目（10JCYBJC12200）;天津市卫生局科技基金资助项目（2012KR09）

摘　　要：目的动态监测创伤性脑损伤（traumatic brain injury，TBI）后大鼠体感诱发电位（somatosensory evoked potential，SEP）和定量脑电图（quantitative electroencephalogram，QEEG）的变化，探讨亚低温对TBI大鼠的神经保护作用及预后判断。方法取健康成年雄性SD大鼠40只，按随机数字表法分为正常对照组（未经任何外加干预措施）、假手术组（只开骨窗，不钻孔）、TBI组（应用液压冲击仪制作中重度TBI模型）和亚低温（TBI后即刻连续给予冰毯物理降温，维持肛温32—35℃，持续6h后复温至37℃），每组10只。各组分别于处理后6，24h、7d监测TBI大鼠SEP和QEEG变化。结果（1）SEP的变化：与TBI组比较，亚低温组24h时伤侧P1波和N1波潜伏期即开始缩短，且差异有统计学意义（P〈0．05），7d时P1波和N1波潜伏期接近假手术组。（2）QEEG的变化：除正常对照组和假手术组，TBI组各时相点均表现为α节律减慢，反应性慢波增多，QEEG相对功率谱值下降或消失；亚低温组反应性慢波减少，伴以少量α波，24h和7d时相对功率谱值升高，尤以24h组升高明显，但仍低于正常对照组（P〈0．05）。结论亚低温治疗可缩短TBI后SEP潜伏期，提高QEEG相对功率谱值，改善神经传导和脑电活动，具有神经保护作用。Objective To investigate the effect of mild hypothermia on neuroprotection and prognosis prediction of rats with traumatic brain injury （TBI） by dynamically monitoring the somatosensory evoked potentials （SEP） and quantitative electroencephalogram （QEEG）. Methods Forty healthy aduh male SD rats were randomly divided into four groups according to random number table, ie, normal control group （ with no intervention ）, sham operation group （ fenestration only, without drilling）, TBI group （fluid percussion was used to produce moderate to severe TBI） , and mild hypothemia group （ice blanket was used immediately after TBI for continuous physical cooling and rectal temperature was main- tained at 32-35℃ and rewarmed to 37℃ 6 hours after the initiation of cooling）, with 10 rats per group. Changes of SEP and QEEG in all groups were monitored at 6, 24 hours, and 7 days after TBI. Results （ 1 ） Compared with TBI group, the latency of SEP waves （ P1 and N1 ） on the injured side in mild hypot- hemia group began to shorten at 24 hours （P 〈 0.05） and were dose to that in the sham operation group at 7 days. （2） Except for normal control group and sham operation group, QEEG in TBI group showed decrease of α rhythm, increase of reactivity slow waves, and decrease or disappearance of QEEG relativepower spectral values at all time points. In mild hypothermia group, the reactivity slow waves were decreased with a small amount of α wave; QEEG relative power spectral values were increased at 24 hours and 7 clays （ especially at 24 hours）, but were still lower than those in normal control group （ P 〈 0.05 ）. Conclusion Mild hypothermia exerts neuroprotective effect through reducing SEP latency, raising rela- tive power speetral values of QEEG, and improving the nerve conduction and brain electrical activity of the injured side.

关 键 词：脑损伤 诱发电位 躯体感觉 脑电描记术 亚低温 

分 类 号：R651.1[医药卫生—外科学]