高压氧对大鼠创伤性脑损伤后血小板膜糖蛋白表达的影响  

Effect of hyperbaric oxygenation on expression of platelet membrane glycoprotein in rats of traumatic brain injury

作　　者：雷箴[1] 王国忠[2] 贾兴元[1] 

机构地区：[1]首都医科大学附属北京朝阳医院医学研究中心,100020 [2]民航总医院氧疗医学科

出　　处：《中华创伤杂志》2014年第4期361-364,共4页Chinese Journal of Trauma

摘　　要：目的探讨高压氧对创伤性脑损伤（traumatic braininjury，TBI）大鼠血小板膜糖蛋白CD31和CD62p表达的影响及意义。方法56只SD大鼠按抽签法随机分为7组：TBI6h组、TBI48h组、TBI96h组、高压氧6h组、高压氧48h组、高压氧96h组以及假手术组，每组8只。建立侧位液压撞击致重度TBI大鼠模型。应用流式细胞术在相应时相点检测各组大鼠血小板膜糖蛋白CD31和CD62p表达。结果TBI6h组、TBI48h组、TBI96h组CD31（30．8±9．0，32．5±9．2，29．0±5．0）和CD62p（34．5±9．1，33．9±7．5，30．4±6．4）表达均明显高于假手术组（18．9±5．5，19．5±6．1，P〈0．05）。高压氧96h组CD31（22．7±5．5）明显低于TBI96h组（29．0±5．0，P〈0．05）；高压氧48h组、高压氧96h组CD62p（26．1±5．8，23．6±5．7）均分别明显低于TBI48h组、TBI96h组（33．9±7．5，30．4±6．4，P〈0．05）。结论TBI大鼠急性期存在血小板活化增强，高压氧对其有一定的抑制性调节作用，有助于抑制TBI后微血栓形成、减轻继发性脑损伤。Objective To investigate the effect and significance of hyperbaric oxygenation in down-regulation of platelet membrane glycoproteins CD31 and CD62p in rats of traumatic brain injury （TBI）. Methods Fifty-six SD rats were randomly distributed into TBI group, hyperbaric oxygenation group, and sham group by the lottery method. Furthermore, TBI group and hyperbaric oxygenation group were subgrouped at 6, 48, and 96 hours. There were 8 rats per group. The rat models of severe TBI were induced by lateral fluid percussion. Levels of CD31 and CD62p were measured in all groups by flow cytometry. Results At 6, 48 and 96 hours, expressions of CD31 （30.8±8.9, 32.5±9.2 and 29.0±5.0） and CD62p （34.5±9.1, 33.9±7.5 and 30.4±6.4） in TBI group were significantly higher than those （ 18.9±5.5, 19.5±6.1 ） in sham group （P 〈 0.05）. At 96 hours, expression of CD31 （22.7±5.5） in hyperbaric oxygenation group was significantly lower than 29.0 ＋ 5.0 in the TBI group （P 〈 0. 05 ）. At 48 and 96 hours, expressions of CD62p （26.1±5.8, 23.6±5.7） in hyperbaric oxygenation group were significantly lower than 33.9±7.5 and 30.4±6.4 in TBI group （ P 〈 0.05 ）. Conclusions Platelet activation is enhanced in the acute phase after TBI. But platelet activation may be relieved with hyperbaric oxygenation, which is conducive to inhibiting microthrombosis and mitigating secondary brain injury after TBI.

关 键 词：脑损伤 高压氧 血小板膜糖蛋白类 血小板活化 

分 类 号：R651.1[医药卫生—外科学]