年轻妇女宫颈上皮内瘤变Ⅰ~Ⅱ级组织中p16^(InK4a)和Ki67的表达及意义  

The Expressions of p16^(InK4a) and ki67 in Cervical Intraepithelial Neoplasia GradeⅠ-Ⅱ in Young Women

作　　者：李思琦[1,2] 董颖[1] 毕蕙[1] 张晓明[1] 陈锐[1] 

机构地区：[1]北京大学第一医院,北京100034 [2]北京积水潭医院,北京100035

出　　处：《实用妇产科杂志》2014年第4期263-265,共3页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:探讨年轻妇女宫颈上皮内瘤变(CIN)Ⅰ~Ⅱ级组织中细胞周期抑制因子p16InK4a蛋白及细胞增殖活性相关抗体Ki67的表达及与病变级别的相关性。方法:选取2010年在北京大学第一医院妇产科门诊就诊、年龄小于35岁,经阴道镜活检病理诊断为CINⅠ及CINⅡ级的病例56例,对其阴道活检组织及宫颈环形电切除术(LEEP)后组织之石蜡切片,进行p16InK4a及Ki67免疫组织化学染色检测其表达。结果:①阴道镜宫颈活检组织的CINⅠ及CINⅡ中p16InK4a阳性表达率分别为30.0%、80.6%,Ki67阳性表达率分别为20.0%,77.8%,CINⅡ级病变组织中p16InK4a及Ki67阳性表达率高于CINⅠ级,差异有统计学意义(P<0.001)。②阴道镜宫颈活检组织标本及LEEP组织标本不同级别CIN病变组织中,p16InK4a及Ki67的阳性表达率存在差异(均P<0.001);p16InK4a及Ki67的阳性表达率与病变级别呈正相关(r=0.644,r=0.645)。结论:p16InK4a及Ki67在年轻妇女CINⅠ~Ⅱ级组织中均有表达,且p16InK4a及Ki67阳性表达与CIN级别相关,可在CIN病理分级中提供参考。Objective：To detect the expressios of p16^InK4a and ki67 in ClN I- Ⅱ in young women and to analyze the relations between their expressions and the grade of the CIN, Methods：56 women （≤〈35 y） with a histologic diagnosis of CIN I - Ⅱ, who attended a colposcopy with cone biopsy in Peking University First Hospital Obstetrics and Gynecology out-patient department in 2010, were included in this study. p16 ^InK4a and ki67 Immunohistochemical staining were performed on all tissues to detect their expressions. Re- sults：①ln punch biopsy tissues： positive expression rate of p16^InK4a in CIN I and CIN Ⅱ were 30. 0% and 80.6%, respectively; positive expression rate of Ki67 in CIN I and CIN Ⅱ were 20.0% ,77. 8%, respective- ly,the positive expression rates of p16^InK4a and Ki67 in CIN Ⅱ were significantly higher than those of CIN [ （ P 〈0. 001 ）. ②ln punch biopsy tissues and LEEP tissues： there were significant differences in positive ex- pression rate of p16 ^InK4a and ki67 among different grade of CIN（ P 〈0. 001 ）, both p16^InK4a and ki67 expres- sion and lesion of CIN have a positive correlation （r = 0. 644, r = 0. 645）. Conclusions： There are both p16 ^InK4a and Ki67 expressions in CIN in young women , and expressions of p16 ^InK4a and ki67 were both posi- tively correlated with the grade of CIN lesion. The results demonstrate their role as markers in supporting the diagnosis of grade of CIN lesion.

关 键 词：细胞周期抑制因子p16^InK4a胁蛋白 细胞增殖活性相关抗体Ki67 年轻妇女 宫颈上皮 内瘤变 

分 类 号：R711.74[医药卫生—妇产科学]