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作 者:陶思美 郑维[1,2] 赵朋超[3] 周伟[1,2] 权春善[1,2] 范圣第[1,2]
机构地区:[1]大连民族学院生命科学学院,大连116600 [2]国家民委教育部重点实验室,大连116600 [3]河南科技大学医学技术与工程学院,洛阳471023
出 处:《中国生物工程杂志》2014年第3期56-60,共5页China Biotechnology
基 金:辽宁省教育厅重点实验室资助项目(LS2010049)
摘 要:Iturin家族环脂肽对于红色毛癣菌等病原真菌具有较强的抑菌活性,有潜力成为治疗皮肤疾病的新型抗真菌药物。解淀粉芽孢杆菌Q-426菌株能够产生环脂肽fengycins和iturin家族的bacillomycin D,但该菌株发酵液具有溶血活性。为确定bacillomycin D是否为引发溶血作用的主要物质,本文利用同源重组基因敲除技术,构建解淀粉芽孢杆菌bmy基因缺失菌株,抑制bacillomycin D的合成,研究对其溶血性及抗菌活性的影响。突变菌株发酵液中未检测到bacillomycin D产生,且发酵液的溶血性及抗菌活性明显减小,表明bacillomycin D与该菌的溶血活性及抑菌活性密切相关。Iturin family of lipopeptides has strong antifungal activity against pathogenic fungi such as Trichophyton rubrum and has potential to become a new antifungal drug currently used against dermatophytes. Bacillus amyloliquefaciens Q-426 could produce fengycins and bacillomycin D ( a member of iturin family), and its fermentation broth shows a hemolytic activity. In order to determine whether bacillomycin D is the main hemolytic substances, a homologous recombinantion gene knockout technology was used to construct strain Q-426- A-bmy. Bacillomycin D was not detected in the Q-426-A-bmy strain fermentation broth, and there was a significant reduction in the hemolysis and an bacillomycin D is closely related to hemolytic tifungal activity in contrast with wild strain Q-426, indicating that activity and antifungal activity of Bacillus amyloliquefaciens Q-426.
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