表没食子儿茶素-3-没食子酸酯抑制脂多糖诱导的巨噬细胞促炎因子TNF-α和IL-1β基因表达  

Epigallocatechin-3-gallate Inhibits LPS-induced Gene Expression of TNF-a and IL-1β in Murine Macrophage

作　　者：张东芳[1] 肖鹏[1] 韩晨露[1] 李卫国[1] 王坤英[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《中国生物化学与分子生物学报》2014年第4期402-408,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：河南省重点科技攻关计划(No.122102310282);河南省教育厅自然科学基础研究计划(No.2010A180011)~~

摘　　要：表没食子儿茶素-3-没食子酸酯（epigallocatechin-3-gallate，EGCG）具有抗氧化、抗癌、抗炎等多种生物学特性，但对巨噬细胞中表达TNF-α及IL-1β的报告尚存在争议．本文旨在探索EGCG对脂多糖（LPS）诱导的小鼠腹腔巨噬细胞和RAW264-7细胞促炎细胞因子Tnf-α和Il-1β基因表达的影响．MTT结果显示，0-100μmol／LEGCG对RAW264．7细胞活力没有影响；实时荧光定量PCR（qRT-PCR）和ELISA分析显示，1ing/LLPS可显著升高小鼠腹腔巨噬细胞和RAW264．7细胞Tnf-α和Il-1βmRNA和蛋白水平，EGCG单独处理对巨噬细胞Tnfα和Il-1β的基因表达与蛋白生成没有影响，但可以抑制LPS诱导的巨噬细胞Tnf-α和Il-1β的基因表达与蛋白生成，并存在剂量依赖效应．上述结果提示，EGCG可以剂量依赖方式抑制LPS诱导的巨噬细胞促炎细胞因子Tnf-α和Il-1β的表达，这可能与EGCG的抗炎效应有关．Epigallocatechin-3-gallate （EGCG） has various pharmacological activities, including anticancer, anti-oxidative and anti-inflammatory effects. However, whether EGCG affects the expression of proinflammatory cytokines of Tnf-α and II-1β in macrophage is still in dispute. This study aimed to characterize the effect of EGCG on lipopolysaccharide （LPS）-induced Tnf-α and II-1β expression in murine peritoneal and RAW264.7 macrophages. The MTT results showed that EGCG did not exert cytotoxicity at tested concentrations of 0 - 100 results showed that 1 mg/L LPS stimulated the μmol/L in RAW264.7 cells. The qRT-PCR and ELISA mRNA and protein levels of Tnf-α and Il-1β, but not EGCG treatment alone. In fact, EGCG inhibited LPS-induced Tnf-α and Il-1β expression and protein secretion in a dose-dependent manner. Our results suggested that EGCG inhibited LPS-induced pro inflammatory gene expression for its anti-inflammatory effects.

关 键 词：表没食子儿茶素-3-没食子酸酯 脂多糖 巨噬细胞 肿瘤坏死因子-α 白介素-1Β 

分 类 号：Q291[生物学—细胞生物学] Q786