银杏提取物对重症急性胰腺炎肺损伤大鼠肺泡中性粒细胞功能的影响  

Effect of Ginkgo biloba Extract on the Function of Alveolar Polymorphonucler Neutrophils in Severe Acute Pancreatitis Rats Complicated with Lung Injury

作　　者：徐晓武[1] 杨小敏 金洲祥[1] 朱少俊[1] 

机构地区：[1]温州医学院附属第二医院普外科浙江温州325027 [2]温州市人民医院病理科浙江温州325000

出　　处：《中国中西医结合杂志》2014年第4期460-465,共6页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的探讨银杏提取物(Ginkgo biloba extract,GBE)对重症急性胰腺炎(severe acute pancreatitis,SAP)合并肺损伤大鼠肺泡中性粒细胞(PMN)功能的影响。方法 48只成年SD大鼠随机分为3组即假手术组、SAP组、GBE治疗组,每组16只。采用5%牛磺胆酸钠逆行胰胆管注射成功制作SAP模型,假手术组仅行十二指肠翻动,GBE治疗组在SAP模型基础上予GBE干预,假手术组和SAP组给予等量生理盐水;分别于术后6h和12h处死大鼠。取肺组织标本进行肺组织损伤评分,检测肺组织湿/干重比(W/D)和肺组织髓质过氧化酶(myeloperoxidase,MPO)活性,经支气管肺泡灌洗分离收集PMN,ELISA法检测支气管肺泡灌洗液(BALF)中性粒细胞弹性蛋白酶(NE)含量,流式细胞术检测PMN表面黏附分子CD11b/CD18双阳性细胞百分率,Western blot法检测肺组织细胞间黏附分子-1(ICAM-1)和NE表达。结果与假手术组比较,SAP组大鼠肺组织出现明显病理组织学损伤,肺组织损伤评分、肺组织W/D、MPO及BALF中NE含量升高,肺组织ICAM-1和NE蛋白表达明显上调,PMN表面黏附分子CD11b/CD18双阳性细胞百分率增加(P<0.01);与SAP组比较,GBE治疗组肺病理组织学损伤显著减轻,上述各项指标均显著下降(P<0.01)。结论 SAP合并肺损伤时,PMN表面黏附分子CD11b/CD18和肺组织ICAM-1表达上调,导致PMN黏附于肺血管内皮细胞,继而激活PMN释放NE,加重肺损伤。GBE可通过下调ICAM-1和CD11b/CD18表达,阻止PMN在肺内黏附和激活,从而减轻肺损伤。Objective To explore the effect of Ginkgo bi/oba extract （GBE） on the function of alveolar polymorphonucler neutrophils （PMN） in severe acute pancreatitis （SAP） rats complicated with lung injury （LI）. Methods Forty-eight adult SD rats were randomly divided into three groups, i.e., the sham-operation group, the SAP group, and the GBE treatment group, 16 in each group. The SAP model was successfully induced by retrograde injection of 5% sodium taurocholate solution into the biliopancreatic duct. Rats in the sham-operation group only received flipping of the duodenum. Those in the GBE treatment group received GBE intervention based on SAP model. Equal volume of normal saline was given to rats in the sham-operation group and the SAP group. Rats were sacrificed at 6 and 12 h after operation respective- ly. The lung tissue was sampled to evaluate the LI score. The wet/dry ratio （W/D） of lung tissues was detec- ted. The activity of myeloperoxidase （MPO） was measured. Alveolar PMN was harvested by bronchoalveolar lavage. The content of neutrophil elastase （NE） in bronchocalveolar lavage fluid （BALF） was measured by enzyme-linked immunoabsorbent assay （ELISA）. The percentage of CD11b/CD18 double positive PMN was detected using flow cytometry. The expression of intercellular adhesion molecule-1 （ICAM-1） and NE protein in the lung tissue was detected by Western blot. Results Compared with the sham-operation group, significant pathologic lesion occurred in the lung tissue of rats in the SAP group~ the pathologic LI score, lung tissue W/D ratio, MPO, and NE content in BALF significantly increased, the expression of ICAM-1 and NE in the lung tissue was obviously up-regulated, and the percentage of CD11b/CD18 double positive PMN significantly increased （~P 〈0.01 ）. Compared with the SAP group, pathological lesion of the lung tissue was obviously attenuated, and the above indices were all significantly declined in the GBE treat- ment group （~P 〈0.01 ）. Conclusions Expression of

关 键 词：银杏提取物 重症急性胰腺炎 肺损伤 中性粒细胞 CD11B CD18 

分 类 号：R657.51[医药卫生—外科学]