利用深度测序技术发掘植物病毒资源  

Application of Next-Generation Sequencing Technology for Plant Virus Identification

作　　者：钱亚娟[1] 徐毅[1] 周琦[2] 周雪平[1] 

机构地区：[1]浙江大学生物技术研究所水稻生物学国家重点实验室,杭州310058 [2]北京出入境检验检疫局检验检疫技术中心,北京100026

出　　处：《中国科学：生命科学》2014年第4期351-363,共13页Scientia Sinica(Vitae)

基　　金：质检公益性行业科研专项(批准号:201310068)资助

摘　　要：第二代测序技术(NGS)因其快速和灵敏的检测特点已成为植物病毒学研究的强有力工具.NGS具有非序列依赖性的优势,能同时检测植物样品中可培养和不可培养的、含量高及含量低的所有的DNA病毒、RNA病毒以及类病毒,它的出现极大地变革和推进了病毒的发现历程.利用该技术已在多种植物上进行了植物病毒的发掘和病原鉴定,取得了非常好的效果.本文围绕NGS及其在植物病毒发掘中的应用研究和前景进行概述和展望.Next-generation sequencing technology （NGS） has been used as a powerful tool for studies on plant pathogens, especially for plant virus identification. NGS is sequence-independent and culture-independent approach, which can be used for detection of RNA viruses, DNA viruses and viroid simultaneously in a plant sample which contains very low titers. Diagnostic for plant viruses is easy by using this revolutionary technology. In the past few years, NGS has been successfully used for survey and identification of viruses in numerous plant species. In this review, we discuss the application and future perspective of NGS in plant virus identification.

关 键 词：NGS 植物病毒 鉴定 

分 类 号：S432.41[农业科学—植物病理学]