流感疫苗壳聚糖脂质体的制备工艺  

Study on Preparation of Chitosan Coated Influenza Vaccine Liposome

作　　者：乔建斌[1] 陈娜[1] 李强[1] 李培辉[1] 晏和贵 鲁卫东[1] 

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500

出　　处：《昆明医科大学学报》2014年第4期38-40,53,共4页Journal of Kunming Medical University

摘　　要：目的在流感疫苗脂质体的基础上制备壳聚糖脂质体载药微粒,建立流感疫苗壳聚糖脂质体的制备工艺.方法采用薄膜分散法制备流感疫苗脂质体,以不同N/P比(壳聚糖中的氮和卵磷脂中的磷比值)制备壳聚糖载药微粒.观察形态并测定其平均粒径、粒度分布、包封率与结合率.通过对比不同N/P比制备壳聚糖载药微粒的包封率与结合率等指标,确定流感疫苗壳聚糖脂质体的制备工艺.结果流感疫苗脂质体形态均呈圆或椭圆形,粒度分布较均匀,壳聚糖包覆后脂质体仍为圆形,平均粒径由2.14μm增加到4.05μm.壳聚糖包覆前后流感疫苗脂质体平均包封率由80.41%变为84.37%.比较6种不同N/P比流感疫苗壳聚糖脂质体的结合率,当N/P为5:1时,为壳聚糖与脂质体孵育的最佳比例,结合率为19.40%.结论壳聚糖包覆后脂质体仍接近圆形,粒径增加,包封率提高,当N/P为5:1时,为壳聚糖与脂质体孵育的最佳比例.Objective To prepare chitosan microparticles based on influenza vaccine liposome, and determine the preparation technology of chitosan liposome vaccine. Methods We prepared chitosan microparticles with different N/P ratio based on influenza vaccine liposomes which were produced by film dispersion method. Then we observed the morphology and determined the average particle size and particle size distribution, entrapment efficiency and the binding rate. By comparing the encapsulation rate and binding rate of different N/P ratio chitosan nanoparticles, the preparation technology of ehitosan liposome vaccine was preliminary determined. Results Influenza vaccine liposomes coated with ehitosan were round or oval with uniformed particle size distribution. The average particle size increased from 2.14 μ m to 4.05 μ m, and the average encapsulation rate increased from 80.41% to 84.37% after coated liposomes with chitosan. By comparing 6 N/P ratio, the ration of 5：1 was the best ratio in terms of binding rate which was 19.40% . Conclusions Chitosan coated liposomes is circular with increased particle size and encapsulation rate. 5：1 ratio of N/P is the optimum proportion concerning the incubation of chitosan and liposome.

关 键 词：流感疫苗 脂质体 壳聚糖 包封率 结合率 

分 类 号：R186[医药卫生—流行病学]