小干扰RNA长期作用乙型肝炎病毒转基因小鼠对机体免疫系统的影响  

Effects of Long-Term siRNA Treatment on the Immune System of HBV Transgene Mice

作　　者：张卫云[1] 石玉玲[1] 孙朝晖[1] 任广立[2] 张蓉[1] 唐荣芝[1] 

机构地区：[1]广州军区广州总医院检验科,广东广州510010 [2]广州军区广州总医院儿科,广东广州510010

出　　处：《生物技术通讯》2014年第2期217-220,共4页Letters in Biotechnology

基　　金：广东省医学科研基金(A2012444)

摘　　要：目的:探讨小干扰RNA(siRNA)长期作用乙型肝炎病毒(HBV)转基因小鼠对机体免疫系统的影响。方法:siRNA表达载体经微静脉注射转染HBV转基因小鼠,设立特异性siRNA组(pSilencer4.1/C2)、PBS对照组和正常BALB/c小鼠组(n=10);分别于注射后1、3、6、9和12个月经其内眦静脉采血,采用散射比浊法测定IgG、IgA、IgM水平的变化,ELISA测定细胞因子IL-2、IL-4、IL-6和IFN-γ,流式细胞术检测小鼠外周血CD4+和CD8+T淋巴细胞亚群。结果:pSilencer4.1/C2长期作用转基因小鼠后可以改善机体的先天免疫反应,转基因小鼠机体内的体液免疫和细胞免疫水平较治疗前均有所上调,高于PBS组,几乎接近正常BALB/c小鼠组。结论:pSilencer4.1/C2长期作用转基因小鼠可以上调机体的先天免疫,可为以后免疫疫苗提供应用时机。Objective： To investigate the effects of long-term small interfering RNA（siRNA） treatment on the im mune system of hepatitis B virus（HBV） transgene mice. Methods： The constructed siRNA expressed vectors were transfected into HBV transgene mice by hydrodynamics-based injection via vena caudalis. Different groups were set including： specificity siRNA group（pSilencer4.1-C2）, PBS group and negative BALB/c mice group（n=lO）. The effect was observed in 1, 3, 6, 9 and 12 months after injection respectively. IgG, IgA and IgM were analyzed by immune nephelometry; IL-2, IL-4, IL-6 and IFN-γ were analyzed by ELISA; CD4＋ and CD8＋ T lymphocyt ewere analyzed by flow cytometry. Results： Our results demonstrated that the innate immune systems of HBV transgenic mice were improved after a long-term treatment with pSilencer4.1-C2. Compared with the PBS group, specifici siR- NA group showed increased levels of index on the cellular immunity and the humoral immunity, almost reached the negative BALB/c mice group. Conclusion： The study showed that the innate immue system of the HBV trans gene mice could be raised with the specific siRNA vetor pSilencer4.1-C2 for a long-term treatment. This will sup ply a potential chance for the usage of HBV vaccine.

关 键 词：小干扰RNA 乙型肝炎病毒转基因小鼠 免疫系统 

分 类 号：R392.1[医药卫生—免疫学] Q78[医药卫生—基础医学]