结核分枝杆菌脂阿拉伯甘露聚糖的提纯及活性初步鉴定  

Purification and Identification of Mycobacterium tuberculosis Lipoarabinomannan

作　　者：戴振华[1] 郭兰芹 冯晓燕[1] 张立[3] 郄霜[1,4] 杨锡琴[1] 修冰水[1] 陈堃[1] 彭瑞云[5] 张贺秋[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]华北石油总医院,河北任丘062552 [3]天津海河医院,天津300350 [4]河北联合大学,河北唐山063000 [5]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《生物技术通讯》2014年第2期230-233,共4页Letters in Biotechnology

基　　金："十一五"国家科技重大专项(20092X10004-718)

摘　　要：目的：提取纯化结核分枝杆菌（MTB）脂阿拉伯甘露聚糖（LAM）。方法：MTB菌体彻底破碎后，去脂，去蛋白，上清液经苯酚萃取，酒精沉淀，得到LAM；以提取的LAM作为包被抗原检测血清中的LAM抗体。结果和结论：提取到LAM抗原，免疫印迹表明，LAM迁移范围相对分子质量为25x10^3-40x10^3，主要集中在35x10。处。在64例肺结核患者中，有43例LAM-ELISA检测阳性（敏感性为67．19％）；在67例健康志愿者中，有64例LAM-ELISA检测阴性（特异性为95．52％）。Objective： To extract and purify lipoarabinomannan （LAM） from Mycobacterium tuberculosis （MTB）. Methods： The MTB was crashed. The lipids and protein were removed, the supernatant was harvested and extract ed with phenol, and precipitated with ethanol, and LAM was obtained. Results ＆ Conclusion： LAM antigen is prepared successfully. By Western blots, the obtained LAM migration was in the range 25-40 kDa, mainly concen trated in the 35 kDa place. Of the 64 tuberculosis patiens, 43 were positive using the LAM-ELISA（sensitivity 67.19%）. Of the 67 healthy volunteers, 64 were negative using the LAM-ELISA（specificity 95.52%）.

关 键 词：结核分枝杆菌 脂阿拉伯甘露聚糖 提纯 

分 类 号：Q502[生物学—生物化学]