高表达SUMO蛋白对人肝星状细胞LX-2纤维化的影响  

Effect of Overexpressing SUMO Protein on Fibrogenesis in Human Hepatic Stellate Cell Line LX-2

作　　者：潘裕华[1] 许晨舒[1] 李樱媚 曹聪[1] 陈孝[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080

出　　处：《今日药学》2014年第3期164-167,共4页Pharmacy Today

基　　金：广东省药学会研究基金(编号:2013A25);广东省重大科技专项(编号:2011A080300004)

摘　　要：目的研究在体外培养的人肝星状细胞LX-2中高表达SUMO蛋白对LX-2纤维化指标的影响。方法采用脂质体瞬时转染法分别将SUMO-1/2/3的高表达质粒转染入LX-2细胞中,分别运用RT-PCR和Western Blot检测α-SMA、CollagenⅠ、MMP-2、TIMP-1的mRNA和蛋白表达量。结果转染质粒48 h后α-SMA、CollagenⅠ的mRNA和蛋白表达水平显著降低(P<0.05),MMP-2、TIMP-1的mRNA表达量亦显著降低(P<0.01),但MMP-2和TIMP-1的蛋白表达水平无明显影响。结论高表达SUMO蛋白能抑制人肝星状细胞LX-2的纤维化过程。Objective To investigate the effect of overexpression of SUMO protein on fibrogenesis in Human Hepatic Stellate Cell Line LX-2 in vitro. Methods LX-2 cells were transfected with SUMO-1/2/3 overexpressing plasmids. Real-time PCR and Western Blot were used to measure the mRNA and protein expression of a-SMA, Collagen I , MMP- 2 and TIMP-1, respectively. Results The mRNA and protein expression of a -SMA and Collagen I were significantly reduced 48 h after transfection of SUMO plasmids. The mRNA expression of MMP-2 and TIMP-1 was also markedly downregulated. However, the protein expression of MMP-2 and TIMP-1 was not affected. Conclusion Overexpression of SUMO protein inhibited the expression of fibrogenesis in LX-2 cells.

关 键 词：SUMO 肝星状细胞 肝纤维化 

分 类 号：R96[医药卫生—药理学]