新生大鼠心肌细胞体外原代培养及鉴定  

Cultivation and Identification of Primary Myocardial Cells from Neonatal Rats

作　　者：汪燕[1] 韦美丹[1] 冯焕村[1] 

机构地区：[1]南方医科大学第三附属医院药剂科,广东广州510630

出　　处：《今日药学》2014年第3期168-170,共3页Pharmacy Today

摘　　要：目的探讨一种较为理想的体外原代心肌细胞培养的方法。方法取新生SD大鼠心脏心尖部，冷胰蛋白酶和II型胶原酶．BSA消化分离单细胞，二次差速贴壁法纯化心肌细胞，倒置相差显微镜和流式细胞仪检测细胞存活率、活力及纯度。结果获得的心肌细胞存活率为90％，纯度达94％以上，自发搏动明显，频率为80—120次／min，且培养前15d细胞搏动频率差异无统计学意义（P〉0．05）。结论本方法可获得较高存活率及纯度的心肌细胞，可满足实验要求。Objective To explore an ideal method for primary culture of cardiomyocytes in neonatal rat. Methods Cardiomyocytes of neonatal rat were isolated in trypsin and collagenase II, and harvested by the second time of adherence. Then the livability, energy and the purity of cardiomyocytes were identified. Results A total of 94% of the cultured cells were myocaridial cells and 90% of them were alive. All the myocaridial cells were beating synchronously, and beating frequency of cells was 80 - 120 beats/min after 3 d. Conclusion This is an effective way to obtain myocardial cells for scientific experimentation.

关 键 词：心肌细胞 体外培养 新生SD大鼠 牛血清白蛋白 

分 类 号：R96[医药卫生—药理学]