HPLC法测定预混剂中恩拉霉素的含量  

Content Determination of Enramycin in Premixes by HPLC

作　　者：曾玉勤[1] 杨亚勇[1] 黄炜乾 程艳 

机构地区：[1]清远职业技术学院 [2]清远容大生物工程有限公司,广东清远511500

出　　处：《今日药学》2014年第3期189-191,共3页Pharmacy Today

基　　金：广东省清远市产学研合作项目(编号:2010C003)

摘　　要：目的采用高效液相色谱法测定饲料中恩拉霉素的含量。方法测定色谱条件为：色谱柱为C18（5um，4．6mm×250mm），流动相为0．05mol／L磷酸二氢钠-乙腈（70：30），检测波长为267nm。结果恩拉霉素A及恩拉霉素B在12．5～200ug/mL范围内线性关系良好，相关系数R。分别为0．9960和0．9945；恩拉霉素A的回收率为96．4％-101．6％，恩拉霉素B的回收率为96．8％～101．2％。恩拉霉素A及恩拉霉素B的RSD分别为0．65％和1．12％。结论该方法线性范围宽、分析时间短、样品前处理简便、定量结果准确、重复性好，为其质量控制提供了依据。Objective To determine the content of Enramycin in feed by high performance liquid chromatographic method. Methods Determination of chromatographic conditions were as follow： column was C18 （5 um, 4.6 mm × 250 mm） , mobile phase was 0.05 mol/L sodium dihydrogen phosphate-acetonitrile （70：30 ,v/v）, detection wavelength was 267 nm. Results Enramycin A and Enramycin B showed a good linear relationship in the range of 12.5 - 200 ug/mL （ra2 =0.996 0, rb2 =0. 994 5）. The recovery rate of Enramycin A, and Enramycin B was 96.4% to 101.6% and 96. 8% to 101.2%. The RSD of Enramycin A and the Enramycin B was 0.65% and 1. 12%. Conclusion This method showed a wide linear range of the determination, a short analysis time, simple pre-treatment of samples, quantitative results and a good reproducibility. It could provide the basis for the quality control.

关 键 词：恩拉霉素 预混剂 高效液相色谱 含量测定 

分 类 号：R512.620.5[医药卫生—内科学]