油藏微生物在人工模拟的多孔介质下的多样性分析  

Diversity Analysis of Microbial Community in Oil Reservoir under Artificial Simulation of Porous Medium

作　　者：刘全全[1] 郑经堂[1] 刘海昌[2] 

机构地区：[1]中国石油大学(华东),山东青岛266580 [2]农业部沼气科学研究所,成都610041

出　　处：《中国沼气》2014年第2期9-14,共6页China Biogas

摘　　要：油藏环境是一个高温、高压、厌氧以及多孔隙结构的极端环境。陶制材料内部密布着各种尺寸的微孔,这些微孔结构为厌氧微生物生长过程提供良好的栖息环境。在微孔结构中,随着微生物菌群的生长,微孔中的氧化还原电位不断降低,这就形成了油藏环境高厌氧的特点;微生物菌群中存在一些能够产气的微生物,产生的气体可在微孔结构中形成一个高压的微环境,由此模拟油藏环境高压厌氧的特点。文章借助亨盖特厌氧操作技术,借助陶质材料疏松多孔的特性,人工模拟油藏地质结构特性。借助变性梯度凝胶电泳技术,分析在人工多孔结构和常规液体培养(无人工多孔结构)的环境中微生物菌群结构多样性差异。研究结果对比显示,在含有多孔结构的培养环境中,菌群结构更为复杂,菌群种类多样性更为丰富。Oil reservoir is an extreme environment ,which is in high temperature, high pressure, anaerobic and porous struc- ture. The ceramic material has all kind size of pores providing a good habitat for anaerobic microbe. The redox potential will decrease in the micro-porous structure with the growth of microorganism. And some microbes are capable of producing gas, which can form a high pressure environment in the micro-porous structure. In this study, the high pressure and anaerobic porous structure of oil reservoir was simulated using the micro-porous ceramic material and Hungate anaerobic technology. The differences of microbial community diversity between the porous structure and conventional liquid culture was analyzed adopting the denaturing gradient gel electrophoresis technology. The result showed that the microflora structure were more complicated under the condition of micro-porous environment, and with more abundant flora species.

关 键 词：油藏 DGGE 多样性 多孔结构 

分 类 号：Q938[生物学—微生物学]