1株聚磷菌株的鉴定及其积累聚–β–羟基丁酸酯的条件  

Identification of a phosphate-accumulating bacteria strain and influencing factors on poly-β-hydroxybutyrate accumulation

作　　者：赵美[1] 金子靖[1] 李博[1] 刘哲君[1] 赵敏[1] 孙艺萍[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《湖南农业大学学报（自然科学版）》2014年第2期202-206,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(J1210053)

摘　　要：从SBR活性污泥中筛选、分离出1株有聚–β–羟基丁酸酯(PHB)积累能力的聚磷细菌(将其命名为"LB4"),通过形态、生理生化及16S rDNA方法分析,鉴定该聚磷细菌为鲍曼不动杆菌(Acinetobacter baumannii)。采用厌氧、好氧交替培养方法,研究影响其积累聚–β–羟基丁酸酯(PHB)的因素。结果表明:在厌氧初始阶段,当pH为8.0,COD为2 000 mg/L,培养温度为35℃时,通过批式补料方式短期限制磷元素对其进行培养,最有利于该菌株积累PHB,PHB的最高积累量可达菌体干重的32.6%。LB4, a phosphate-accumulating strain with the ability of poly-β-hydroxybutymte（PHB） granules accumulation, was isolated from activated sludge of SBR in treatment equipment of wastewater. Through the analysis from morphological, biochemical characteristics, and identification using 16S rDNA sequence approach, LB4 was proved to be a subspecies ofAcinetobacter baumannii. The influence factors for PHB accumulation were investigated by alternative cultivation of anaerobic-aerobic cycle. The results showed that the favourite conditions for the strain to accumulate PHB at the beginning of anaerobic phase was at temperature 35 ℃, pH 8.0, COD 2 000 mg/L, and with short-term phosphorus inhibition, the maximum accumulation of PHB could reach to that of 32.6% dry weight of the strain.

关 键 词：聚磷细菌 聚-β-羟基丁酸酯(PHB) 鲍曼不动杆菌 

分 类 号：S216.3[农业科学—农业机械化工程]