海洋来源真菌草酸青霉(Penicillium oxalicum)次级代谢产物的分离与鉴定  

Isolation and identification of the secondary metabolites from the marine-derived fungus Penicillium oxalicum

作　　者：王培乐[1] 李丹毅[1] 谢磊睿[1] 华会明[1] 李占林[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第4期252-255,300,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(21002064;20872094)

摘　　要：目的研究海洋来源真菌草酸青霉(Penicillium oxalicum)的次级代谢产物。方法采用重结晶、硅胶柱色谱、Sephadex LH-20柱色谱、开放ODS柱色谱等方法进行分离纯化;根据理化性质及光谱数据确定化合物的结构。结果分离得到10个化合物,分别鉴定为N-acetyl-hydrazinobenzoic acid(1)、N-[2-cis-(4-hydroxyphenyl)ethenyl]formamide(2)、p-hydroxyphenylacetamide(3)、penipanoid A(4)、penipanoid C(5)、2-(4-hydroxybenzyl)quinazolin-4(3H)-one(6)、oxaline(7)、meleagrin(8)、(3R,4R)-3,4,8-trihydroxy-3,4-dihydro-1(2H)-naphthalenone(9)和(3R,4R)-3,4,6,8-tetrahydroxy-3,4-dihydro-1(2H)-naphthalenone(10)。结论化合物1为新天然产物,化合物9、10为首次从青霉属真菌中分离得到。Objective To study the secondary metabolites from a marine-derived fungus Penicillium oxali- cum. Methods The compounds were isolated and purified by recrystallization, silica gel, Sephadex LH- 20, and ODS column chromatography, and their chemical structures were elucidated by their physico-chemical properties and spectral analysis. Results Ten compounds were obtained and identified as N-acetyl-hydrazino- benzoic acid （ 1 ）, N- [ 2-cis- （ 4 -hydroxyphenyl ） ethenyl ] formamide （ 2 ）, p -hydroxyphenylacetamide （ 3 ）, penipanoid A （ 4 ）, penipanoid C （ 5 ）, 2- （ 4-hydroxybenzyl ） quinazolin-4 （ 3 H） -one （ 6 ）, oxaline （ 7 ）, meleagrin （8）, （3R, 4R ） -3,4,8-trihydroxy-3,4-dihydro-1 （2H） -naphthalenone （ 9 ）, and （ 3R, 4R ） -3,4,6,8-tetra- hydroxy-3,4-dihydro-1 （2H）-naphthalenone （10）. Conclusions Compound 1 is a new natural product, and compounds 9-10 are isolated from Penicillium genus for the first time.

关 键 词：草酸青霉 次级代谢产物 生物碱 二氢萘酮 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]