羧甲基-β-环糊精用于6种抗胆碱类药物的毛细管电泳拆分  

Enantiomeric separation of six anticholinergics drugs with carboxymethy-β-cyclodextrin by capillary electrophoresis

作　　者：胡艳丽[1] 孙嘉仪 袁晓薇[1] 孙铁民[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第4期266-271,330,共7页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273476)

摘　　要：目的建立高效毛细管电泳法分离6种抗胆碱类药物对映体。方法采用未涂层熔融石英毛细管柱,以羧甲基-β-环糊精(carboxymethy-β-cyclodextrin,CM-β-CD)为手性选择剂,磷酸盐缓冲液作为背景电解质。结果在最佳条件下,托吡卡胺、氢溴酸后马托品、甲溴后马托品、阿托品和溴甲阿托品的分离度(R)分别为5.13、6.29、4.67、4.85和4.59,消旋山莨菪碱的分离度(R)分别为1.13、6.92和2.58。结论 CM-β-CD对6种抗胆碱类药物对映体均有较好的对映体选择性。Objective To establish a method of high performance capillary electrophoresis for the chiral separations of six anticholinergics drugs. Methods The separations of enantiomers were performed in an untreated fused-silica capillary tube,using CM-β-CD as chiral selectors,buffer of 30 mmol.L^-1 NaH2PO4 as BGE. Results Under the optimal conditions, the resolutions of tropicamide, homatropine hydrobromide, homatropine methyl bromide, atropine and atropine methobromide were 5.13,6.29,4.67,4.85 and 4.59, respectively, the resolutions of anisodamine were 1.13,6.92 and 2.58 respectively. Conclusions CM-β-CD is highly enantioselective for the six anticholinergics drugs. The method is simple, fast with high reproducibility.

关 键 词：毛细管电泳 羧甲基-β-糊精 抗胆碱类药物 手性分离 

分 类 号：R917[医药卫生—药物分析学]