金边龙舌兰超氧化物岐化酶的提取与特性研究  

Study on extraction and properties of SOD from Agave americana variegata marginata hort leaf

作　　者：杨就锦 陈冰[1,2] 李嘉亮[1] 苏玉天 谢施文 盘夏媚 

机构地区：[1]湛江师范学院化学科学与技术学院,广东湛江524048 [2]广东高校新材料工程技术开发中心,广东湛江524048

出　　处：《化学研究与应用》2014年第4期497-500,共4页Chemical Research and Application

基　　金：2012年国家级大学生创新创业训练计划项目(教高司函[2013]8号)资助;湛江师范学院第五届学生课外科研课题(湛师团委[2012]138号)资助;2011年广东省科技计划项目(粤科规划字[2011]97号)资助

摘　　要：金边龙舌兰经匀浆、丙酮沉淀、葡聚糖凝胶过滤等步骤,纯化获得2个超氧化物歧化酶（ SOD） I和Ⅱ。该SODI和Ⅱ适应pH范围分别在4-11和4-10,pH分别为7和8时活性最高；均在60℃以下稳定,60℃能够保持较高的活力；分别在272．5和274．5 nm处有最大紫外光吸收；敏感性抑制试验证明,其活性均被氰化钾和过氧化氢所抑制,但不受氯仿-乙醇影响,与Cu·Zn-SOD相似。Superoxide dismutases（ SOD）Ⅰ andⅡwere purified from Agave americana variegata marginata Hort,which was homog-enate,precipitation by acetone and gel filtration on Sephadex G column. The enzymes were relatively stable at temperatures below 60℃. At 60℃,the enzymes had the most activity. At pH ranging from 4-10 and 4-11,the enzymes were relatively stable. At pH 7 and 8,the enzymes had the most activity,respectively. They had the absorption maximums of ultraviolet absorption spectroscopy at 272. 5 and 274. 5 nm,respectively. They were inhibited by potassium cyanide and hydrogen peroxide,but not influcend by CHCl3-C2 H5 OH,which was similar to copper-and zinc containing SOD.

关 键 词：金边龙舌兰 超氧化物歧化酶 丙酮沉淀法 葡聚糖凝胶过滤 

分 类 号：O629.8[理学—有机化学]