水稻黑条矮缩病毒P9-1在烟草表皮细胞中自我互作并形成包涵体  

Rice black streaked dwarf virus P9-1,self-interacts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants

作　　者：谭子龙[1,2] 孙丽英[2] 陈剑平[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院病毒学与生物技术研究所/浙江省植物有害生物防控国家重点实验室培育基地/农业部植物保护与生物技术重点开放实验室/浙江省植物病毒重点开放实验室,浙江杭州310021

出　　处：《浙江农业学报》2014年第2期365-370,共6页Acta Agriculturae Zhejiangensis

基　　金：973项目(2010CB126203);国际科技合作计划(2012DFA30900);国家自然科学基金(31071660);浙江省植物病毒与组培产业重点领域创新团队(2009R50032)

摘　　要：水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)是斐济病毒属的成员,由昆虫介体灰飞虱传播,能够在植物宿主和昆虫介体中复制。同其他斐济病毒成员一样,RBSDV的复制与装配是在细胞质病毒包涵体结构即毒质结构里进行的。RBSDV有10条基因组dsRNAs(S1—S10),其第9条片段(S9)的第一个阅读框(ORF)编码的蛋白P9-1是形成毒质结构的框架蛋白。将P9-1与绿色荧光蛋白(GFP)融合后,在本氏烟草的表皮细胞中单独表达,通过激光共聚焦扫描显微镜观察发现P9-1可以形成包涵体结构;利用双分子荧光互补(BiFC)实验证明了P9-1具有自我互作的能力,并形成包涵体结构。Rice black-streaked dwarf virus （RBSDV）, a member of the genus Fijivirus, is transmitted in nature by the small brown planthopper （Laodelphax striatellus） and can replicate in cells of both host plants and insect vector . RBSDV, like other members of the family Reovirus , is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms.It has genomic segments of 10 dsRNAs （S1-S10） and P9-1 encoded by the first open reading frame in the ninth segment （ S9） of the genomic RNAs is an essential protein for the formation of viroplasms.Fused with green fluorescent protein （GFP）, P9-1 was able to form inclusion bodies when expressed alone in Nicotiana benthamiana epidermal cell.Bimolecular fluorescence complementation （BiFC） assay suggested P9-1 had the ability of self-interaction and formed inclusion bodies .

关 键 词：水稻黑条矮缩病毒 包涵体 自我互作 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]