CLIP-170在细胞内定位与发挥功能的分子基础  

Molecular Basis of the Cellular Localization and Function of CLIP-170

作　　者：孙晓东[1] 高金珉[1] 刘敏[1] 李登文[1] 周军[1] 

机构地区：[1]南开大学生命科学学院遗传学和细胞生物学系,天津300071

出　　处：《南开大学学报（自然科学版）》2014年第1期80-86,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：构建了带有绿色荧光标记的CLIP-170不同结构域的表达质粒,检测了CLIP-170全长对微管形态的影响及其不同结构域在细胞内与微管相互作用的情况.发现不仅2个CAP-Gly结构域对CLIP-170定位于微管很重要,其coiled-coil结构域和锌指模序也可以使CLIP-170部分定位于微管上.Plasmids expressing different domains of CLIP-170 tagged with green fluores- cence protein were constructed. The effect of full-length CLIP-170 on microtubule morphology and the interaction of different domains of CLIP-170 with microtubules was analyzed. The two CAP-GIy domains of CLIP-170 were critical for its localization on microtubules and the coiled- coil domain and the zinc finger motif of CLIP-170 could also mediate its partial localization on microtubules.

关 键 词：CLIP-170 微管 微管结合蛋白 绿色荧光蛋白 定位 

分 类 号：Q786[生物学—分子生物学]