巴马火麻仁总木脂素酰胺类提取物对小鼠急性肝损伤的保护作用  

Protective effect of lignanamides extract from Fructus Cannabis of Bama on acute hepatic injury in mice

作　　者：刘军[1] 蔡霈 蔡光明[1] 王艳华 

机构地区：[1]中国人民解放军302医院全军中药研究所,北京100039 [2]广西巴马常春藤生命科技发展公司,北京101102

出　　处：《中南药学》2014年第3期228-230,共3页Central South Pharmacy

基　　金：国家发改委专题基金资助项目(编号:国发[2009]-260号)

摘　　要：目的研究广西巴马产火麻仁中提取分离获得的火麻仁总木脂素酰胺类提取物(LFC)对CCl4致小鼠急性肝损伤的保护作用。方法昆明种小鼠,以LFC低、中、高(60、120、180 mg·kg-1)剂量连续给药7 d后,分别采用腹腔注射0.1%CCl4的橄榄油溶液(10 mL·kg-1)建立小鼠急性肝损伤模型,16 h后,杀鼠取材,分别检测小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,肝脏中丙二醛(MDA)、超氧化物歧化酶(SOD)含量,光学显微镜下观察肝组织病理学变化。结果 CCl4模型组肝功能明显异常,ALT、AST显著升高,肝组织变性、坏死严重。经LFC预防性治疗后,血清中ALT、AST活性显著降低(P<0.01)、肝组织中MDA含量显著降低(P<0.01),SOD活力增加;肝脏组织学观察证实,LFC能明显改变受损组织的病理学变化。结论 LFC对CCl4致小鼠急性肝损伤均具有较好的保护作用。Objective To observe the protective effect of lignanamides extract from Fructus Cannabis（LFC） on acute hepatic injury induced by carbon tetrachloride in mice. Methods KM mice were pretreated with LFC low dose（60 mg·kg- 1）, middle dose（120 mg·kg- 1）, and high dose（180 mg·kg- 1） for 7 days. Mouse models of acute liver injury were established by the treatment of CCl4. After 16 hours, the activities of AST and ALT in the serum, MDA level and the activity of SOD in the liver were measured by the detection kits according to the manufacturer protocols. The liver histopathological characters were observed under the microscope. Results At each dose of LFC, the activities of AST and ALT in the serum（P 0.01） and MDA level in the liver（P 0.01） were significantly reduced, the activity of SOD in the liver was significantly increased compared with that in the CCl4 model group. Under the microscope, liver injury induced by CCl4 was greatly decreased in each LFC dose group. Conclusion LFC has protective effect on liver damage induced by CCl4 in mice.

关 键 词：火麻仁 木脂素酰胺类 CCL4 急性肝损伤 

分 类 号：R965[医药卫生—药理学]