七十味珍珠丸对局灶性脑缺血大鼠脑组织中SOD、CAT、MDA的影响  

Effect of Rannasangpei on the SOD,CAT and MDA in Brain Tissues of Rats with Local Cerebral Ischemia

作　　者：甄丽芳[1] 罗远带[2] 黄福开[2] 胡贤达 邵杰[2] 尚颖[1] 刘亚丽[1] 杜文兵[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]中国藏学研究中心北京藏医院,北京100029

出　　处：《世界中医药》2014年第3期347-349,354,共4页World Chinese Medicine

基　　金：国家重大财政专项"藏医心脑血管病临床研究与药物研发"(编号:1981020421)

摘　　要：目的:观察七十味珍珠丸对永久性大脑中动脉阻塞(Permanent Middle Cerebral Artery Occlusion,PMCAO)大鼠脑组织中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)及丙二醛(Maleic dialdehyde,MDA)含量的影响,初步探讨其脑保护作用机制。方法:取SD大鼠用线栓法制备PMCAO模型。观察七十味珍珠丸对大鼠脑梗死体积、脑组织中超SOD、CAT及MDA含量的影响。结果:与PMCAO模型组比较,七十味珍珠丸高、低剂量组大鼠脑梗死体积都有不同程度的减小(P<0.01或P<0.05),脑组织中SOD、CAT含量都有所升高,MDA含量有不同程度下降(P<0.01)。结论:七十味珍珠丸可能通过有效地增强内源性抗氧化酶清除自由基的能力、减轻脑组织脂质过氧化损伤来实现脑保护作用的。Objective： To explore the effect of Rannasangpei on the SOD , CAT and MDA in the brain tissues of rats with permanent mid- dle cerebral artery occlusion （ PMCAO）, and to study its cerebral protective mechanism. Methods： SD rats were made into local cerebral ischemic models with the method of permanent middle artery occlusion. The effects of Rannasangpei on the volume of cerebral infarction in rats and the changes of SOD, CAT and MDA contents in their brain tissues were observed. Results ：The infarct volumes of rats in two Rannasangpei intervention groups were obviously decreased compared with the PMCAO model group （P 〈 0. 01 orP 〈 0. 05 ） ;compared with the PMCAO model group, the SOD and CAT contents in brain tissues of the rats in the high and low dose Rannasangpei groups were all significantly increased, the MDA contents were decreased in different degrees （P 〈 0. 01 ）. Conclusion：Rannasangpei has significant protective effect on cerebral ischemic injury in rats, and its protective mechanism may come from effectively enhancing ability of the en- dogenous antioxidant enzyme to scavenge the free radicals, and relieving lipid peroxidation damage of brain tissue.

关 键 词：七十味珍珠丸 局灶性脑缺血损伤 氧化应激 

分 类 号：R285.5[医药卫生—中药学]