丹红注射液调节大脑中动脉阻塞大鼠血清氨基酸代谢谱的实验研究  

Research on Danhong Injection Adjusting Metabolic Spectrum of Serum Amino Acid in Cerebral Infarction Rats

作　　者：郭志丽[1] 朱妍[1,2] 肖红斌[3] 王忠[1] 

机构地区：[1]中国中医科学院,北京100700 [2]陕西步长制药有限公司,西安712000 [3]中国科学院大连化学物理研究所,大连116023

出　　处：《世界中医药》2014年第3期355-358,共4页World Chinese Medicine

摘　　要：目的：利用代谢组学手段检测大脑中动脉阻塞（Middle Cerebral Artery Occlusion，MCAO）模型大鼠血清中氨基酸含量，微观上阐明氨基酸注射液的疗效以及部分作用机制。方法：采用邻苯二甲醛（0-Phthalaldehyde，OPA）柱前在线衍生反相高效液相色谱法，采用荧光检测，梯度洗脱，建立了血清中氨基酸代谢谱的相对定量分析方法，测定36例大鼠血清中的15种氨基酸含量。流动相A为10mol／LNa2HP04-Na2807缓冲液（pH=7．95），B为乙腈-甲醇-水（45：45：15，V／V／V），线性梯度洗脱，流动相B在6min内由5％增加至17．2％，6～35min内由17．2％增加至52％。结果：1）该法在35min之内分离出15种氨基酸；2）经主成分分析，3个组在空间上得到很好地分离，氨基酸代谢谱发生了一定的变化；3）利用SPSS方差分析，有6个氨基酸在分离各组有统计荤意义。结论：丹红注射液通过部分调节MCA0摇动通路改善脑梗死大鼠血清部分氨基酸的代谢趋势。Objective：To determine the content of amino acid to clarify the curative effect and the functioning mechanism of DanHong injection on microscopic. Methods： OPA reversed phase high performance liquid chromatography （ HPLC ）, precolumn derivatization method fluorescence detection, gradient elution were used. Mobile phase A was 15mol/L Na2 HPO4- Na2 B07 buffer （pH = 7.95）, B was acetonitrile - methanol - water （45：45：15, V/V/V）. As for linear gradient elution, mobile phase B in 6 min increased from 5 % to 17. 2%, increased from 17.2% to 52% in 6-35 min. Results：l ） Fifteen kinds of amino acids were isolated within 35 min; 2） using princi- pal component analysis, three groups were well separated; 3 ） using the SPSS analysis of variance, there were six amino acids in the sep- aration between groups was statistically significant. Conclusion： Danhong injection can improve metabolic trend of amino acid in cerebral infarction rats serum through adjusting perturbed pathways

关 键 词：高效液相色谱 氨基酸代谢组学 脑梗死 丝氨酸代谢 

分 类 号：R285.5[医药卫生—中药学] R743.3[医药卫生—中医学]