妊娠晚期羊水过少胎膜与胎盘绒毛上皮DNA-RNA反应观察  

Observation of DNA-RNA reaction in the amnion and placenta of OR patients

作　　者：石凡华[1] 

机构地区：[1]泰安市妇幼保健院,山东泰安271000

出　　处：《泰山医学院学报》2014年第1期24-26,共3页Journal of Taishan Medical College

摘　　要：目的比较晚发羊水过少(OR)患者与正常妊娠的胎膜和胎盘DNA-RNA组织化学反应的不同及其意义。方法取足月分娩正常量羊水和OR患者的羊膜和胎盘各15例,常规石蜡制片,分别进行组织学常规染色和DNA-RNA反应,光镜观察并进行形态计量学分析。结果与正常组比较,OR羊膜细胞及胎盘滋养层细胞出现明显的形变及固缩,细胞核染色质浓缩深染、边缘化,染色质分割碎解等细胞凋亡的形态学特征,两者形态计量结果有显著差异(P<0.01)。结论羊膜及胎盘上皮细胞凋亡、老化、功能减退是OR发生的重要因素之一。Objective ：To Compare the differences in DAN-RNA reaction to amnion and placenta between OR and normal pregnancy and study the meaning. Methods ： Fifteen cases of amniotic membrane and placental tissue with normal a- mount amniotie fluid and oligohydramnios were in each group. The routine paraffin section had HE staining and DAN-RNA reacting, and the slices were observed under light microscope and some morphomatric indexes were analyzed. Results ： Com- pared with normal the amniotic ceils and the villous syncytiophoblast had deformation and displayed ladder bands character- istic of internucleosomal DNA of apoptotie ceils. There was significant difference between the two groups （ P 〈 0. 01 ）. Con- clusion ： Aging and apoptosis of amniotic ceils and villous syncytiophoblast resultant disfunction is one of the important fac- tors of the oligohydramnios.

关 键 词：羊水过少 羊膜 胎盘 DNA-RNA反应 

分 类 号：R714.2[医药卫生—妇产科学]