OsPIN1a Gene Participates in Regulating Negative Phototropism of Rice Roots  

作　　者：XU Hua-wei MO Yi-wei WANG Wei WANG Hai WANG Zhong 

机构地区：[1]College of Agriculture,Henan University of Science and Techenology [2]College of Life Science,Shaoxing University [3]College of Life Science,Anhui Agricultural University [4]College of Bioscience and Biotechnology,Yangzhou University

出　　处：《Rice science》2014年第2期83-89,共7页水稻科学（英文版）

基　　金：supported by the grants from the National Natural Science Foundations of China(Grant Nos.31071353 and 31100197);the Anhui Provincial Natural Science Fund of Youth,China(Grant No.1308085QC50);the Fund of Provincial Excellent Young Talents in Universities and Colleges,China(Grant No.2012SQRL057)

摘　　要：The complete open reading frame of OsPINla was amplified through reverse transcriptase- polymerase chain reaction （RT-PCR） based on the sequence deposited in GenBank to explore the relationship between the auxin efflux protein OsPINla and the negative phototropism of rice roots. Sequencing results showed that the GC content of OsPINla was 65.49%. The fusion expression vector pCAMBIA-1301-OsP/N1a：：GFP containing the OsPINla gene and a coding green fluorescent protein （gfp） gene was constructed. The fusion vector was transferred into onion epidermal cells by Agrobacterium tumefaciens transformation. The transient expression of OsPINla-GFP was mainly located in the nucleus and cell membrane. Moreover, the transgenic plants were obtained by Agrobacterium-mediated genetic transformation. Molecular detection performed by using PCR and β-glucuronidase staining showed that the target construct was integrated into the genome of rice. The negative phototropic curvatures of the transgenic rice roots were higher than those of the wild type. Similarly, the expression levels of OsPINla in the transgenic plants were considerably higher than those in the wild-type plants. These results suggest that OsPINla is crucial in the negative phototropic curvature of rice roots.The complete open reading frame of OsPINla was amplified through reverse transcriptase- polymerase chain reaction （RT-PCR） based on the sequence deposited in GenBank to explore the relationship between the auxin efflux protein OsPINla and the negative phototropism of rice roots. Sequencing results showed that the GC content of OsPINla was 65.49%. The fusion expression vector pCAMBIA-1301-OsP/N1a：：GFP containing the OsPINla gene and a coding green fluorescent protein （gfp） gene was constructed. The fusion vector was transferred into onion epidermal cells by Agrobacterium tumefaciens transformation. The transient expression of OsPINla-GFP was mainly located in the nucleus and cell membrane. Moreover, the transgenic plants were obtained by Agrobacterium-mediated genetic transformation. Molecular detection performed by using PCR and β-glucuronidase staining showed that the target construct was integrated into the genome of rice. The negative phototropic curvatures of the transgenic rice roots were higher than those of the wild type. Similarly, the expression levels of OsPINla in the transgenic plants were considerably higher than those in the wild-type plants. These results suggest that OsPINla is crucial in the negative phototropic curvature of rice roots.

关 键 词：RICE OsPINla green fluorescent protein transient expression negative phototropism 

分 类 号：S511[农业科学—作物学]