颈椎后纵韧带骨化症与BMP-2、BMP-4基因多态性的关联性研究  

Relationship Between the Gene Polymorphisms of BMP-2 and BMP-4 with Ossification of Posterior Longitudinal Ligament of the Spine in a Chinese population

作　　者：周耀 魏威[2] 陈春悦[2] 叶杨[2] 

机构地区：[1]浙江省奉化市中医医院,奉化315500 [2]浙江省中西医结合医院

出　　处：《浙江中西医结合杂志》2014年第4期296-297,348,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省医药卫生科技计划项目(No.2010KY168);浙江省宁波市科技计划项目(No.2013C50023)

摘　　要：目的探讨颈椎后纵韧带骨化症(OPLL)与BMP-2基因第3外显子的rs79417223和BMP-4基因第4外显子的rs147822607两个SNP位点的相关性。方法选取47例浙江地区汉族颈椎后纵韧带骨化症患者,以100名正常个体作为对照组,采用PCR+Sanger DNA测序,统计分析2个位点基因型的分布信息。结果 BMP-2基因第3外显子的rs79417223G→A患者、BMP-4第4外显子的rs147822607A→C患者与正常组呈现明显差异(P<0.05,P<0.01)。结论 BMP-2基因第3外显子的rs79417223G→A、BMP-4基因、第4外显子的rs147822607A→C,这2个多态性位点与OPLL的发病具有强烈相关性。Objective To explore the relationship between 2 single nucleotide polymorphisms（SNPs） of BMP-2 and BMP-4 and ossification of posterior longitudinal ligament of the spine（OPLL）in a Chinese population. Methods Forty-seven sporadic OPLL patients and 100 healthy controls in Zhejiang area were recruited for association analy-sis. The SNP regions of BMP-2 and BMP-4 genes were amplified by PCR and Sanger sequencing. Genotype fre-quencies of the SNPs were compared between the cases and control subjects with SPSS 13.0 software. Results Both Of the rs79417223 G→A site in the exon 3 of BMP-2 and the rs147822607 A→C site in the exon 4 of BMP-4,the patient and normal control groups showed significant differences（P〈0.05,P〈0.01）. Conclusions The rs79417223 and rs147822607 have a strong correlation to OPLL.

关 键 词：颈椎后纵韧带骨化症 单核苷酸多态性 易感基因 BMP-2基因 BMP-4基因 

分 类 号：R681.5[医药卫生—骨科学]