抗稻瘟病内生真菌DW22次级代谢产物醇提取方法的研究  

Studies on Alcohol Extraction Method of Secondary Metabolites from Rice Blast Resistant Endophytic Fungi DW22

作　　者：陈珣[1] 杨镇[1] 肖军[1] 丑静[1] 龚娜[1] 王娜[1] 王红[1] 肇莹[1] 杨涛[1] 

机构地区：[1]辽宁省农业科学院微生物工程中心,沈阳110161

出　　处：《吉林农业科学》2014年第2期65-67,共3页Journal of　Jilin Agricultural Sciences

基　　金：辽宁省科技攻关项目(2011215005);"百千万人才工程"入选项目择优资助项目(2012921065)

摘　　要：从辽宁省西北地区根系土壤中分离得到DW22号内生真菌,液体培养后,分别用20%、40%、60%、80%、100%乙醇提取其次级代谢产物,依次编号为22-1、22-2、22-3、22-4、22-5。进行抗稻瘟病菌的抑制活性检测和方差及差异显著性分析。结果表明:20%乙醇提取DW22号内生真菌次级代谢产物对抑制稻瘟病菌有较好的效果。Secondary metabolites from DW22, an endophytic fungus isolated from root-zone soil col- lected in Northwest of Liaoning, were extracted with 20%, 40%, 60%, 80% and 100% ethanol, respectively. The ethanol extracts were numbered with 22-1, 22-2, 22-3, 22-4 and 22-5 in order. Inhibitory activities to Magnaporthe grisea were detected, and the variances and significant differences were analyzed. The results showed that 20% ethanol extract of secondary metabolites from DW22 endophytic fungus had better inhibit- ing effect to Magnaporthe grisea.

关 键 词：内生真菌 次级代谢产物 醇提取方法 稻瘟病 抑制活性检测 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]