金银花复方制剂对新城疫病毒感染雏鸡外周血液免疫学变化的影响  

Effect of Compound Honeysuckle Preparation on Peripheral Blood Immunological Changes of Chickens Infected with Newcastle Disease Virus

作　　者：张瑞莉[1] 于群[1] 梁尧[1] 孟巍[1] 刘瑞[1] 葛铭 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国家禽》2014年第7期22-26,共5页China Poultry

基　　金：黑龙江省自然科学基金项目(C201222);黑龙江省博士后科研启动资助基金项目(LBH-Q11314)

摘　　要：为研究金银花复方制剂对新城疫病毒(NDV)感染雏鸡外周血液免疫学变化的影响,采用免疫荧光试验、流式细胞术、间接ELISA法和放射免疫法对NDV感染雏鸡、金银花复方制剂治疗雏鸡的外周血液淋巴细胞数量、免疫球蛋白及细胞因子含量进行检测。结果显示,雏鸡感染NDV后,其外周血液CD4+、CD8+T淋巴细胞和B淋巴细胞数量及IgG、IgM、白细胞介素(IL-2)含量均较未感染雏鸡明显降低,γ-干扰素(IFN-γ)含量较未感染雏鸡明显升高,提示NDV感染能够引起雏鸡外周血液免疫功能紊乱;金银花复方制剂能够显著提高感染雏鸡外周血液CD4+T淋巴细胞和B淋巴细胞数量及IgG、IgM、IL-2含量,降低IFN-γ含量,表明该复方制剂能够改善NDV感染雏鸡外周血液的免疫功能。To investigate the effect of compound honeysuckle preparation on peripheral blood immunological changes of chickens infected with Newcastle disease virus （NDV）, immunofluorescence assay, flow cytometry, indirect enzyme linked immunosorbent assay and radioimmunoassay were used to detect the number of lymphocytes and content of immunoglobuliu and cytokine in the peripheral blood of chickens indefected with NDV and treated with compound honeysuckle preparation. The results showed that the numbers of CD4＋,CDs＋T lymphocyte and B lymphocyte,the contents of IgG, IgM and interleukin-2 （IL-2）in the peripheral blood of chickens infected with NDV were significantly lower than those of uninfected chickens. The content of Interferon-3, （IFN-3,）was heightened obviously. The results indicated that peripheral blood immune function of infected chickens was disordered. But the numbers of CD4＋T lymphocyte and B lymphocyte, the contents of IgG,IgM and IL-2 were increased and the content of IFN-3, was decreased in the peripheral blood of treatment chickens. It signed that the traditional Chinese medicine honeysuckle compound preparation could improve the blood immune function of chickens infected with NDV.

关 键 词：金银花复方制剂 新城疫病毒 雏鸡 外周血液免疫 

分 类 号：S858.315.3[农业科学—临床兽医学]