50%戊唑·咪鲜胺微乳剂有效成分的高效液相色谱检测  

Determination of the Active Ingredients inTebuconazole and Prochloraz 50% Micro-emulsion by High Performance Liquid Chromatography

作　　者：尹兰香[1] 黄琼辉[1] 刘丽英[1] 江晴[1] 

机构地区：[1]福建省农产品质量安全检验检测中心,福建福州350003

出　　处：《福建农业学报》2014年第3期291-295,共5页Fujian Journal of Agricultural Sciences

摘　　要：建立了在同一色谱条件下测定混剂中戊唑醇和咪鲜胺含量的方法。试样用甲醇溶解，以甲醇∶乙腈∶水（体积比35∶30∶35）为流动相，流速1.0 mL · min-1，使用XTerra RP-18柱和二极管阵列检测器（220 nm ），对50％戊唑·咪鲜胺微乳剂（30％戊唑醇＋20％咪鲜胺）中的戊唑醇和咪鲜胺进行反相高效液相色谱分离和测定，外标法定量。结果表明：戊唑醇在101.01～1010.08 mg · L -1质量浓度范围呈线性响应，相关系数为0.9999，相对标准偏差为0.19％，平均加标回收率为101.14％；咪鲜胺在99.06～990.85mg · L -1质量浓度范围呈线性响应，相关系数为0.9998，相对标准偏差为0.13％，平均加标回收率为101.22％。该方法简便、快速、分离效果好，可用于产品的质量控制和检测分析。The determination method of the active ingredients in tebuconazole and prochloraz 50% micro-emulsion （ME） was established by High Performance Liquid Chromatography （HPLC） .Samples were dissolved by methanol and then determined by the reversed phase HPLC .The HPLC conditions were methanol-acetonitrile-water （35∶30∶35 ,by vol） as mobile phase ,flow-rate was 1.0 mL · min-1 ,XTerra RP-18 column ,Photodiode Array （PDA） detector and detection wave length at 220nm . The external standard method was employed for quantitative analysis .The results showed that the linear relationship between the peak area and concentration was in the range of 101.01 to 1 010.08 mg · L -1 （r=0.999 9） for tebuconazole ,and 99.06 to 990.85 mg · L -1 （r= 0.999 8） for prochloraz ,respectively .Meanwhile ,the RSD were 0.19% and 0.13% ,and the average recoveries were 101.14 and 101.22% .Our results suggested that the method with simple ,rapid and good separating effect ,can be used for the quality control and detection of products .

关 键 词：戊唑醇 咪鲜胺 高效液相色谱 

分 类 号：S482.3[农业科学—农药学]