从12SrRNA基因序列研究中国蛙科24种的进化关系  

EVOLUTIONARY RELATIONSHIPS AMONG CHINESE RANID FROGS INFERRED FROM MITOCHONDRIAL DNA SEQUENCES OF 12S rRNA GENE

作　　者：江建平[1] 周开亚[1] 

机构地区：[1]南京师范大学生物多样性与分子进化研究室,南京210097

出　　处：《动物学报》2001年第1期38-44,共7页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金!资助项目 (39970 0 94)

摘　　要：测定了中国蛙科动物 2 4种和蟾蜍科的中华大蟾蜍线粒体 12SrRNA基因长约 40 0bp片段的序列。采用邻接法 ,对其数据的系统发生分析表明 2 4种可分成 3个支系 :第一个支系包括棘腹蛙、合江棘蛙、棘胸蛙、大头蛙、虎纹蛙、倭蛙、泽陆蛙共 7种 ,其中 3种棘蛙组成一个单系 ;第二个支系由 3种湍蛙 ,即戴云湍蛙、华南湍蛙和武夷湍蛙组成 ,应属于湍蛙亚科 ;第三支系包含余下的 14种蛙 ,其中 5种林蛙和 4种臭蛙分别组成一个单系 ;2种侧褶蛙先聚合后 ,再与弹琴水蛙聚合 ,2种粗皮蛙首先聚合在一起 ,再与由林蛙组成的单系群聚合。第三支系首先与第二支系相聚 ,然后它们与第一支系形成姐妹群 ,即由蛙亚科的 7个物种组成的第一支系 ,不与蛙亚科另 14个物种组成的第三支系直接聚合 ,表明第一支系的物种可能不属于蛙亚科。此外 ,分子证据提示把陆蛙属、大头蛙属、虎纹蛙属、棘蛙属、臭蛙属、粗皮蛙属和侧褶蛙属从原来的蛙属分出有其合理性。Approximately 400 base pairs of the mitochondrial 12S rRNA gene from 24 species of Chinese ranid frogs (Table 1) were sequenced. Phylogenetic analyses of these data using neighbor joining method showed that these frogs were divided into three lineages. The first lineage (BCL 78%) contains 7 species, Fejervarya limnocharis, Hoplobatrachus rugulosus, Limnonectes fujianensis, Paa boulengeri, P.spinosa, P.robertingeri, and Nanorana pleskei , of which 3 species of Paa forming a monophyletic group (BCL 99%). The second lineage (BCL 99%) comprises 3 species, Amolops daiyunensis, A. ricketti , and A. wuyiensis , coresponding to the subfamily Amolopinae. The rest 14 species belong to the third lineage (BCL 61%), of which 5 species of Rana and 4 species of Odorrana forming a monophyletic group respectively. Two species of Pelophylax cluster together before they cluster with the genus Hylarana . Two species of Rugosa form a clade before they cluster with the monophyletic group of 5 species of Rana . The second and third lineage cluster at BCL=85%, and a sister group relationship between the cluster and the first lineage is recognized. In other words, the first lineage comprising 7 species of the subfamily Raninae and the third lineage comprising 14 species of the subfamily Raninae do not cluster directly. This result suggests that the frogs of the first lineage are probably excluded from the subfamily Raninae. In addition, the molecular evidence supports the establishment of ranid genera Fejervarya, Limnonectes, Hoplobatrachus, Paa, Odorrana, Rugosa and Pelophylax . They were separated from the genus Rana which now represent only Brown frogs.

关 键 词：蛙科 分子系统学 MTDNA 12SRRNA基因 进化关系 两栖动物 

分 类 号：Q959[生物学—动物学]