大鼠冠状动脉平滑肌细胞电活动的实验研究  

THE EXPERIMENTAL STUDY OF ELECTRICAL ACTIVITY OF SMOOTH MUSCLE CELLS OF THE RAT CORONARY ARTERY

作　　者：王福文[1] 徐淑兰[1] 刘志向[2] 柴象枢[2] 

机构地区：[1]山东省医学科学院药物研究所,济南250062 [2]山东省医学科学院基础研究所,济南250062

出　　处：《山东生物医学工程》2000年第1期22-26,共5页Shandong Journal of Biomedical Engineering

基　　金：国家自然科学基金!重点资助项目 (No .2 92 391 50 )

摘　　要：应用先进技术细胞内微电极的方法研究了大鼠冠状动脉平滑肌细胞的静息膜电位 (Em)及其对血管活性物质KCl、ACh、NE的反应性。结果表明 ,大鼠冠状动脉平滑肌细胞静息膜电位稳定 ,其均值为 -54.38± 2 .36mv ,KCl和ACh均可引起膜电位去极化 ,且呈剂量依赖式特点 ;NE对膜电位无明显影响。结论　同一动物的不同血管或不同动物的同一血管反应性存有明显的异质性。In this paper,the resting membrane potential(Em) of smooth muscle cells of the rat coronary artery was studied by advanced technique——intracellular microelectrode method.The effects of potassium chloride(KCl),acetylcholine(Ach),norepinephrine(NE) on membrane potential were observed,respectively.The results showed that the resting Em was steady,mean values for Em were -54.38±2.36mv;both KCl and ACh caused depolarization of Em in a concentration-response fashion.NE have no effect on Em.

关 键 词：冠状动脉 血管平滑肌 膜电位 乙酰胆碱 去甲肾上腺素 血管反应性控制 

分 类 号：R543[医药卫生—心血管疾病]