银杏叶提取物对大鼠高糖高胰岛素下视网膜Müller细胞的保护作用  

Effect of EGB761 in protecting cultured Muller cells under high glucose and high insulin condition

作　　者：楼继先[1] 胡昕倩[1] 李韧[1] 张志勇[1] 王美钗[1] 

机构地区：[1]浙江医院,浙江杭州310013

出　　处：《浙江实用医学》2014年第1期1-4,78,共5页Zhejiang Practical Medicine

基　　金：浙江省医药卫生科技计划项目(2011KYB019)

摘　　要：目的观察银杏叶提取物(extract of ginkgo bilobaleaf,EGB761)在高糖高胰岛素作用下对视网膜Müller细胞活性的改变及其保护作用。方法用30mmol/L葡萄糖和(或)200U/L的胰岛素处理大鼠视网膜Müller细胞48小时,分为高糖高胰岛素组:200U/L胰岛素+30mmol/L葡萄糖;高胰岛素组:200U/L胰岛素;对照组:普通低糖型DMEM培养(含葡萄糖5mmol/L)。提前0.5小时加入60μg/mL、120μg/mL的EGB761,观察对各组细胞的保护作用,48小时后用MTT法测定视网膜Müller细胞的活性。结果高糖高浓度胰岛素处理后Müller细胞活性降低,与对照组相比差异有统计学意义(P<0.05)。而60或120μg/mL的EGB761能上调高浓度葡萄糖和高胰岛素处理后的视网膜Müller细胞的活力,且在单纯高胰岛素组中更佳,与无EGB761处理组相比差异有统计学意义(P<0.05)。结论高浓度葡萄糖和高胰岛素在短期内可使视网膜Müller细胞的活性降低,一定浓度的EGB761可减轻高糖高胰岛素对视网膜Müller细胞的损伤。Objective To investigate the activity of cultured retinal M＆#252;ller cell under high glucose and high insulin condition ,and study the effects of EGB761 M＆#252;ller cells’ activity .Methods M＆#252;ller cells from rat retina were collected and cultured in vitro .They were treated with glucose 30 mmol/L and（or） insulin 200U/L for 48 hours .They were divided into three groups ：high glucose and high insulin group with insulin 200U/L and glucose 30mmol/L;high insulin and low glucose group with insulin 200U/L and glucose 5mmol/L;control group with glucose 5mmol/L and no insulin .Different concentrations of EGB761 were added to the medium in all groups and investigated the effects of EGB761 M＆#252;ller cells’ activity .There were three concentrations ：0μg/mL ,60μg/mL ,120μg/mL .After 48 hours incubation ,the ac-tivity of M＆#252;ller cells evaluated by MTT . Results After 200U/L insulin and glucose 30mmol/Ltreatment , the activity of M＆#252;ller cells de-creased dramatically as compared with that of control group （ P＆lt;0.05） .After EGB761 treatment in high insulin and high glucose group ,the activity of M＆#252;ller cells were increased compared with those without EGB761 treatment （ P＆lt;0.05） .Conclusion High concentration of glu-cose and high insulin could decrease the activity of M＆#252;ller cells in a short time .After treatment of EGB761 ,the activity of M＆#252;ller cells could recover .

关 键 词：视网膜MULLER细胞 银杏叶提取物 胰岛素 葡萄糖 EGB761 

分 类 号：R587.1[医药卫生—内分泌] R774.1[医药卫生—内科学]