6个虾种基因组DNA多态性分析  

ANALYSIS OF GENOMIC DNA POLYMORPHISMS IN SHRIMP GROUP

作　　者：许玉德[1] 孙晟[2] 

机构地区：[1]厦门大学生命科学学院,361005 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2001年第1期9-11,46,共4页Marine Sciences

摘　　要：采用RAPD方法检测了罗氏沼虾 (Macrobrachiumrosenbergii)、绿须虾 (Aristeusvirilis)、长毛对虾 (Penaeuspenicillatus)、日本对虾 (P.japonicus)、斑节对虾 (P.monodon)和周氏新对虾(Metapenaeusjoyneri)等6个虾种的基因组DNA的多态性。用20个随机引物扩增得到492个DNA片段 ,根据这些片段的共享度计算出遗传距离并构建系统树。所得结果从DNA水平上反映出虾类在科属种不同分类阶元亲缘关系的远近 。Genomic DNA polymorphisms in six species shrimp (Macrobrachium rosenbergii, Aristeus virilis, Penaeus penicillatus, P. japonicus, P. monodom and Metapenaeus joyneri) were detected using randomly amplified polymorphic DAN(RAPD) method. Amplifications with 20 primers gave 492 reproducible fragments. Index of genetic similarity(F) was calculated. The value of (1-F) was used to evaluate genetic distances between species and to construct phylogenetic tree. These RAPD analysis is consistent with extant taxonomic system of shrimp group. Therefore, overall results revealed phylogenetic relationship of differential taxonomic class of shrimp group on genomic DNA.

关 键 词：虾类 基因组DNA 随机扩增多态性DNA 物种 亲缘关系 

分 类 号：Q959.223.5[生物学—动物学]