羊水ABH血型物质测定与ABO血型基因分型  

Detection of amniotic fluid ABH blood group substances and ABO blood type gene classification

作　　者：陈江[1] 逯心敏 郭渝[1] 胡伟[1] 

机构地区：[1]四川省宜宾市第二人民医院检验科,四川宜宾644000

出　　处：《重庆医学》2014年第11期1302-1303,1307,共3页Chongqing medicine

基　　金：四川省卫生厅资助项目(20100583);宜宾市科技局资助项目(2010SF011)

摘　　要：目的通过检测羊水ABH血型物质和序列特异性引物-聚合酶链反应（PCR—SSP）基因技术检测胎儿羊水细胞ABO血型基因型，鉴定胎儿ABO血型。方法选取妊娠16～25周的孕妇53例，抽取羊水，利用间接凝集实验测定羊水ABH血型物质；将羊水细胞进行分离，提取羊水细胞DNA，运用PCR—SSP技术分析其ABO血型基因型。结果16例羊水标本为非分泌型，占30．2％，37例羊水标本为分泌型，占69．8％；48例羊水标本通过PCRSSP方法检测出了AB0血型的基因型。经基因鉴定的胎儿羊水细胞ABO血型与羊水分泌型ABH血型物质检测结果一致。结论PCR—SSP技术可以准确地检测胎儿羊水细胞的AB（）血型。Objective To detect amniotic fluid ABH blood group substances and ABO blood group genotype by the polymerase chain reaction with sequence-specific primers（PCR-SSP） to increase the prenatal diagnosis of fetal ABO blood group. Methods 53 pregnant women with gestational age 16 - 25 weeks were selected. Amniotic fluid was extracted for detecting ABH blood group substances by the serological indirect agglutinating reaction; the amniotic fluid ceils were separated for extracting DNA. Tben the PCR SSP technique was adopted to analyze the ABO blood group genotypes. Results 16 specimens of arnniotic fluid were non-se- creting type phenotype（30.2 % ） and 37 specimens of amniotic fluid were secreting type phenotype（69.8 % ） ;48 specimens of amni- otic fluid were detected out the ABO blood group genotype by the PCR-SSP method. ABO blood group of fetal amniotie fluid cells by the gene identification was consistent to the detection results of amniotic fluid secreting type ABH blood group substances. Con- clusion The PCR-SSP technique can accurately detect the fetal amniotic fluid cells ABO blood group.

关 键 词：ABO血型系统 羊水 细胞 序列特异性引物-聚合酶链反应 ABH血型物质 

分 类 号：R457.1[医药卫生—治疗学]