补体C5a对肾移植排斥反应中IL-17产生的调节作用  

Regulation effect of complement C5a on interleukin-17 during renal allograft rejection

作　　者：李蜀婧[1] 郑权友 袁刚[1] 霍文谦[1] 张克勤[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所泌尿外科,重庆400042

出　　处：《重庆医学》2014年第11期1331-1334,共4页Chongqing medicine

基　　金：国家自然科学基金面上资助项目(81170695;81370846)

摘　　要：目的探讨肾移植排斥反应中促炎性细胞因子白细胞介素17(IL-17)和补体活化产物C5a的表达情况,以及C5a对IL-17表达的可能调节作用。方法流式细胞术(FCM)检测肾移植患者外周血中IL-17+T细胞的频率和C5a刺激后人肾小管上皮细胞系中的HK2细胞IL-17的表达情况;ELISA检测肾移植患者血清C5a水平的变化;免疫组织化学检测并比较正常肾组织和发生排斥反应移植肾组织中IL-17的表达和补体C5b-9的沉积;免疫细胞化学检测HK2细胞在重组C5a刺激前、后IL-17表达的差异。结果同种异基因肾移植术后,肾移植患者外周血中IL-17+T细胞百分率明显升高,患者血清C5a水平较术前显著增加(P<0.01);发生排斥反应的肾组织中IL-17表达与C5b-9的沉积较正常肾脏组织均有明显的上调,二者呈正相关;加入重组的C5a刺激后,HK2细胞IL-17的表达水平显著上调。结论肾移植排斥反应中补体活化产物C5a可能对IL-17的产生起正向调节作用。Objective To investigate the expression of proinflammatory cytokine interleukin-17（IL-17） and complement cleavage fragment CSa and the regulation effect of C5a on the expression of IL-17 during renal allograft rejection. Methods The frequency of IL-17＋ T cell in peripheral blood and the expression of IL 17 in renal tubular epithelial cells（HK2） after C5a stimulation in renal transplant recipients were measured by flow cytometry and the changes of serum C5a level was detected by ELISA, respectively. Im- munohistochemistry was applied to detect and compare the expression of IL-17 and the deposition of C5b-9 in normal renal tissues and renal tissues with allograft rejection. The difference of IL-t7 expression in HK2 cells before and after the recombinant C5a stimulation was detected by immunocytochemistry. Results Both the percentage of IL 17＋ T cells and serum CSa levels were sig- nificantly increased after the allogeneic renal transplantation. Compared with normal renal tissues,both the deposition of C5b 9 and the IL-17 expression in renal tissues with allograft rejection was remarkably up regulated,which showed the positive correlation be- tween them. The expression of IL-17 in HK2 was obviously up-regulated by the recombinant CSa stimulation. Conclusion CSa may positively regulate the expression of IL-17 by tubular epithelial cells during the renal allograft rejection.

关 键 词：补体C5A 白细胞介素17 肾移植 移植物排斥 

分 类 号：R699.2[医药卫生—泌尿科学]