黄疣海参皂苷hillaside A和hillaside B的体外抗真菌及抗肿瘤活性  

Study on In Vitro Antifungai and Antitumor Activities of Hillaside A and Hillaside B from Sea Cucumber Holothuria nobilis

作　　者：张佳佳[1] 朱可奇[2] 巫军 王开义 

机构地区：[1]浙江医药高等专科学校,浙江宁波315200 [2]浙江省宁波市第一医院,浙江宁波315010 [3]宁波生物医药技术有限公司,浙江宁波315800

出　　处：《中国药业》2014年第8期20-22,共3页China Pharmaceuticals

基　　金：浙江省自然科学基金;项目编号:Y2110082;宁波市自然科学基全;项目编号:2012A610258;浙江省中医药科技计划资助;项目编号:2011ZB125

摘　　要：目的阐明黄疣海参hillaside A和hillaside B的抗肿瘤及体外抗真菌活性。方法对黄疣海参的总提取物采取硅胶柱色谱及半制备高效液相色谱(HPLC)法等分离手段进行活性成分追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以磺酰罗丹明B蛋白染色(SRB)法评价化合物的抗肿瘤活性。结果分离得到了2个新的三萜皂苷元类化合物,命名为hillaside A和hillaside B。2个新化合物均显示了较强的体外抗真菌及抗肿瘤活性。结论研究为研制新的抗肿瘤药物提供了先导化合物,为充分开发利用我国的海洋生物资源提供了一定的物质基础和科学依据。Objective To illuminate the in vitro antifungai and antitumor activities of hillaside A and hillaside B from sea cucumber Holothuria nobilis. Methods The total extract from sea cucumber Holothuria nobilis was performed the trace separation of the active ingredients by adopting the silica gel column chromatography and the semipreparative HPLC. Their chemical structures were identified by the physicochemical properties and spectroscopic methods. Their antitumor activities were evaluated by the SRB method. Results Two new triterpene glycosides were obtained and their structures were identified, named as hillaside A and hillaside B. Two new saponins showed the strong in vitro antifungai and antitumor activities. Conclusion This research provides the lead compounds for studying the new antitumor drugs and a certain material basis for full exploitation and utilization of the marine biological resources in our country.

关 键 词：黄疣海参 hillaside A hillaside B 抗肿瘤 抗真菌 

分 类 号：R282.77[医药卫生—中药学]