无血清诱导脂肪间充质干细胞向雪旺细胞分化的实验研究  

Serum Free Medium Induces Adipose Derived Stem Cells into Schwann Cells

作　　者：张秀霞[1] 蒋良福[2] 

机构地区：[1]温州医科大学附属第二医院急诊科,浙江温州325027 [2]温州医科大学附属第二医院手外科,浙江温州325027

出　　处：《实用骨科杂志》2014年第4期322-325,共4页Journal of Practical Orthopaedics

基　　金：浙江省自然科学基金资助(Y2100253);温州市科技计划项目(Y20130203)

摘　　要：目的探索无血清诱导脂肪间充质干细胞分化为雪旺细胞的方法。方法取大鼠双侧睾丸周围脂肪组织,分离培养原代脂肪间充质干细胞,CD90、CD44和CD45流式鉴定。将脂肪间充质干细胞分成两组,实验组无血清诱导培养,对照组有血清诱导培养。采用雪旺细胞化学诱导因子逐步诱导2周,比较形态学变化以及细胞免疫荧光S-100和GFAP阳性率。结果分离的脂肪间充质干细胞相关的CD90和CD44表达阳性,CD45表达阴性。诱导后两组形态学相似,实验组S-100、GFAP阳性率与对照组比较无明显差异(P>0.05)。结论无血清诱导培养可作为诱导脂肪间充质干细胞向雪旺细胞分化的方法。Objective To explore the method adipose-derived mesenchymal stem cells into Schwann cells.Methods The adipose tissues surrounding testes of rats were harvested for isolating and culturing primary adipose-derived mesenchymal stem cells.CD90,CD44 and CD45 were detected by flow cytometry.The adipose-derived mesenchymal stem cells were divided into two groups.The experimental group non-serum culture,and the serum culture served as control group.Both groups were gradually induced with Schwann cell inducing factor for two weeks.Then the morphological changes and immunofluorescence positive rate of S-100 and GFAP cells were compared between the experimental group and control group.Results The CD90 and CD44 expression was positive in isolated adipose-derived mesenchymal stem cells,while the CD45 expression was negative.Similar morphology were found in both groups after induction.The S-100 positive rate and GFAP positive rate no significant difference between the experimental group and the control group（P ＞ 0.05）.Conclusion Non-serum-induced culture can be used to induce adipose mesenchymal stem cells into Schwann cell.

关 键 词：脂肪间充质干细胞 无血清 雪旺细胞 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]