培养软骨移植修复兔生长板缺损  

REPAIR OF GROWTH PLATE DEFECTS OF RABBITS WITH CULTURED CARTILAGE TRANSPLANTA-TION

作　　者：王建[1] 杨志明[1] 解慧琪[1] 

机构地区：[1]华西医科大学附属第一医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2001年第1期53-56,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家基础研究发展规划 (973)项目!(G19990 5 430 8) ;国家自然科学基金!重点项目 (39830 10 0 )

摘　　要：目的　将体外培养软骨移植修复兔生长板缺损 ,防止生长板缺损早闭造成的肢体发育畸形。方法分离收集 1月龄兔关节软骨细胞 ,经离心管内培养形成软骨。将培养 2周软骨植入 6周龄兔胫骨上端生长板缺损区 ,于 4、16周时对术肢行 X线摄片、组织学及免疫组织化学等检查。结果　兔胫骨上端生长板缺损区移植培养软骨 4周时 ,胫骨无明显畸形发生 ,组织学检查显示生长板缺损由软骨充填 , 型胶原免疫组织化学染色阳性。 16周时移植侧胫骨仍无明显畸形 ,组织学检查显示生长板已近闭合。而对照侧胫骨发生严重畸形 ,生长板闭合。结论培养软骨异体植入兔生长板缺损 ,可替代缺损的生长板软骨组织 ,维持肢体正常生长 ,防止肢体发育畸形。Objective To prevent early closure of growth plate and developmental deformities of limbs by allografts of cultured cartilages into growth plate defects of rabbits. Methods Chondrocytes isolated from articular cartilage of 1 month rabbits formed cartilage after cultivation in centrifuge tubes. The cartilages cultured for two weeks were implanted into growth plate defects of proximal tibiae of 6 weeks rabbits. At 4th and 16th weeks, X ray, histologic and immunohistochemical examination were performed. Results The tibiae had no marked deformities after 4 weeks of operation. Histologic examinations showed that the defects were filled with cartilage. Immunohistochemical results of type Ⅱ collagen were positive. The tibiae with allografts of cultured cartilages had no evident deformities after 16 weeks of operation. Histologic examination showed nearly closure of growth plates. On the contrary, the tibiae on control side formed severe deformities and growth plate were closed. Conclusion Allograft of cultured cartilages into growth plate defects may replace lost growth plate tissues, maintain normal growth of limbs and prevent developmental deformity.

关 键 词：培养 软骨 移植 生长板 组织工程 

分 类 号：R687.3[医药卫生—骨科学]