金霉素对畜禽养殖废水厌氧生物处理及细菌多样性的影响  

Effect of Chlortetracycline on Bacterial Diversity and Anaerobic Biological Treatment for Livestock Wastewater

作　　者：陈峻峰[1] 颜智勇[1] 邵继海[1] 舒鹏[1] 王杰[1] 文树龙 

机构地区：[1]湖南农业大学资源环境学院,湖南长沙410128

出　　处：《净水技术》2014年第2期76-80,85,共6页Water Purification Technology

基　　金：环保部公益性行业科研专项(201109024);水体污染控制与治理科技重大专项(2009ZX07211-001);湖南省研究生科研创新项目(CX2013B302)

摘　　要：该文研究了不同金霉素浓度下畜禽养殖废水厌氧生物处理系统的COD去除率和产甲烷量,运用PCR-DGGE及差异DNA片段回收克隆测序的方法分析了金霉素对该厌氧生物处理系统细菌多样性的影响。结果表明随着金霉素浓度的增加,畜禽养殖废水的COD去除率和产甲烷量均会受到抑制,产甲烷量差值从6.9%扩大到29.2%,COD去除率从90.89%降至86.96%,而对照组COD去除率达到94.46%。PCR-DGGE分析表明金霉素胁迫能够显著降低畜禽养殖废水厌氧生物处理系统中的细菌多样性,细菌群落丰富度值从0.913降至0.652,而对照组的丰富度值为1。COD removal rate and methane yield of livestock wastewater at different concentration of chlortetracycline were studied. The influence of bacterial diversity was analyzed by PCR - DGGE, cloning and sequencing. The results indicate that with the increase of chlortetraeycline concentration, the removal rate of COD and methane yield are both restricted. Difference values of methane yield expand from 6.9 % to 29.2 % and COD removal rate falls to 86.96 % from 90.89 %, while that of the control group is 94.46 %. Results of PCR- DGGE indicate that with the increase of chlortetracycline concentration, bacterial community richness values fall to 0. 652 from 0.913, while that of the control group is 1, which means that chlortetracyeline decreases the bacterial diversity of livestock wastewater.

关 键 词：16S rDNA 聚合酶链式反应 变性梯度凝胶电泳 畜禽养殖废水 系统发育分析 金霉素 

分 类 号：TU992[建筑科学—市政工程]