氯化甲基汞对发育阶段大鼠脑核因子kappaBDNA结合活性的影响  

Effects of methyl mercury chloride on nuclear factor-kappaB DNA binding activities of nuclear protein extracts from developing rat cerebra and cerebella

作　　者：董丽波[1] 李志超[1] 毕晓颖[1] 凌玲[1] 郭丽[1] 

机构地区：[1]白求恩医科大学毒理教研室,长春130021

出　　处：《卫生研究》2001年第1期7-9,共3页Journal of Hygiene Research

基　　金：国家自然科学基金!(No.39770 6 5 5 )

摘　　要：为探讨氯化甲基汞 (MMC)对发育大鼠脑组织核因子 kappa B(NF- k B) DNA结合活性的影响 ,采用凝胶移位法可见发育大鼠的大、小脑核蛋白提取物与 k B探针结合 ,对照和实验组均有 2条结合带。出生后 3天大鼠的大、小脑核蛋白提取物中 NF- k B DNA结合活性高于出生后 7天者。提示在发育大鼠脑中 NF- k B对发育起一定作用。宫内接触 MMC后出生 3和 7天的大脑核蛋白提取物中 NF- k B 和 NF- k B DNA结合活性低于对照组 ,而小脑中的 NF- k B 和 NF- k B DNA结合活性则高于对照组。在体外结合反应体系中 ,随着 MMC剂量的增高可增强大、小脑核蛋白提取物中 NF- k B DNA结合活性。提示接触MMC后大、小脑神经细胞反应能力不同 ,小脑中 NF- k B被激活。The effects of methyl mercury chloride (MMC) on DNA binding activities of nuclear factor kappaB(NF-kB)in developing rat cerebella and cerebra were investigated with electrophoretic mobility shift assays(EMSAs). The bindings of NF-kB in nuclei of rat cerebra and cerebella to kB probes showed two bands on gel shift in both control and experiment groups. NF-kB Ⅰ and NF-kB Ⅱ DNA binding activities of nuclear protein extracts from rat cerebra exposed to MMC in uterus, was lower than control groups on postnatal day 3 and 7, while that from rat cerebella was higher than control groups. The results suggested that the reactive abilities of neural cell to MMC between cerebra and cerebella were different. In binding reaction mixture, the quantities of MMC increased with the increase of NF-kB DNA binding activities of nuclear protein extracts.

关 键 词：氯化甲基汞 核因子-KAPPAB DNA 结合活性 发育脑 

分 类 号：R994.3[医药卫生—毒理学]