Analysis on Quantitative Trait Loci for Resistance to Rice False Smut under Different Environmental Conditions  

不同环境下水稻稻曲病抗性位点检测(英文)

作　　者：李余生[1] 杨娟[1] 黄胜东[1] 王才林[1] 

机构地区：[1]江苏农科院粮食作物研究所,江苏省优质水稻工程技术研究中心,江苏南京210014

出　　处：《Agricultural Science & Technology》2014年第3期449-452,473,共5页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31071397);the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(12)1003)~~

摘　　要：A recombinant inbred line （RILs） population with 157 lines derived from an inter-subspecies cross of Daguandao （japonica）/IR28 （indica） by the single seed de-scent method was used to detect quantitative trait loci （QTLs） conferring resistance to rice false smut caused by Ustilaginoidea virens（Cooke） Takahashi in Nanjing and Yangzhou. The disease rate index of the two parents and 157 RILs caused by rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software. Eight QTLs control ing false smut resis-tance were detected on chromosomes 1, 2, 4, 8, 10, 11 and 12, respectively, with the phenotypic variance of 8.6%-22.5%. There were five QTLs detected in Nanjing and Yangzhou, respectively, and only two QTLs were found in both two years, the phenotypic variation was explained by individual QTL ranged from 18.0% to 18.9% for these two QTLs, and the additive effects of these two QTLs contributed to the 8.0%-14.6% decrease of disease index and therefore the disease resistance increased. The direction of the additive effects at six loci qFsr1, qFsr2, qFsr8, qFsr10a, qFsr11 and qFsr12 coincided with that predicted by phenotypes of the parents, and the IR28 al eles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao al eles at qFsr4 and qFsr10b. Both qFsr10a and qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection （MAS） program.利用157个家系组成的大关稻（japonica）/IR28（indica）重组自交系（Recombinant inbred lines，RIL）群体，采用高效引发稻曲病人工接种方法，以病情指数作为稻曲病的表型值。在南京、扬州分别鉴定了亲本及 RILs对水稻稻曲病的抗性。利用 QTL Cartographer 软件，对水稻稻曲病抗性基因进行检测分析。两个环境下共检测到 qFsr1、qFsr2、qFsr4、qFsr8、qFsr10a、qFsr10b、qFsr11、qFsr12等8个 QTL，分别位于第1、2、4、8、10、11和12染色体上，贡献率在8.6%~22.5%之间。其中，南京检测到qFsr1、qFsr4、qFsr10a、qFsr11、qFsr12等5个位点；扬州检测到 qFsr2、qFsr8、qFsr10a、qFsr10b、qFsr11等5个位点，qFsr10a、qFsr11在两个环境下中均被检测到，对性状的解释率在18.0%~18.9%之间，使病情指数下降8.0%~14.6%,提高了抗病性。根据抗性位点加性效应方向，在qFsr1、qFsr2、qFsr8、qFsr10a、qFsr11和qFsr12位点上，亲本 IR28存在抗稻曲病的增效等位基因，大关稻具有减效等位基因，而位点 qFsr4、qFsr10b的抗性效应来源正好相反。 qFsr10a、qFsr11及其附近的标记可望在稻曲病抗性分子标记辅助选择育种中加以应用。

关 键 词：RICE Rice false smut Resistance locus 

分 类 号：S511[农业科学—作物学]