福建省HIV-1病毒基因亚型分布的研究  

Genetic Subtyping Analysis of HIV-1 Strains in Fujian Province

作　　者：陈舸[1] 严延生[1] 翁育伟[1] 郑兆双[1] 郑健[1] 

机构地区：[1]福建省卫生防疫站，福州 350001

出　　处：《中国公共卫生》2001年第1期31-32,共2页Chinese Journal of Public Health

基　　金：福建省卫生厅青年科学研究基金立项课题

摘　　要：目的　了解福建省HIV 1各亚型的流行情况 ,帮助分析其传染来源和判断流行趋势。方法　采集福建省检出的 13例HIV 1感染者的外周血单核细胞 (PBMCs) ,提取核酸后用套式聚合酶链反应技术进行扩增HIV 1膜蛋白 (env)基因C2 V3区目的核酸片段 ,应用异源双链泳动分析法 (HMA)进行HIV 1亚型的分析。结果　 13份标本PCR扩增均阳性 ,亚型分析结果表明福建省存在HIV 1B亚型和E亚型。结论　HIV 1B亚型和E亚型可能是福建省流行的主要亚型 ,主要经性途径由境外传入 ,且经性传播途径在人群中流行。异源双链泳动分析法可作为HIVObjective To investigate the epidemiology of HIV 1 in Fujian Province,in order to identify the source of HIV 1 transmission and analyze the trend of HIV 1 epideic.Methods The nuclear acids were extracted from peripheral blood mononuclear cells(PBMCs) collected from 13 HIV 1 infected individuals found in Fujian,and DNA fragments(the C2 V3)region,about 782bP)of HIV 1 env gene was amplified by nested PCR,the HIV 1 genetic subtypes were then assayed by heteroduplex mobility assay(HMA).Results Show that 13 samples were determined to be positive by PCR amplification,HIV 1 subtype B and E were found in Fujian.Conclusion HIV 1 subtype B and E may be dominant in Fujian Province,subtype B and E were mainly transmitted into Fujian from abroad and spreaded via sexual transmission route among populations.HMA might be a useful screening method for HIV 1 molecular epidemiological analysis.

关 键 词：人类免疫缺陷病毒1型 基因亚型 异源双链泳动分析法 分子流行病学 

分 类 号：R512.91[医药卫生—内科学]