养阴益气活血冲剂对沙土鼠脑缺血时去甲肾上腺素的影响  

Influence of “Yang Yin YiQiHuoXue Granules”on Norepinephrine During Jird Ischemia

作　　者：蔡宇 白海波 万海同 唐风德[4] 

机构地区：[1]江中制药集团博士后工作站,江西南昌330077 [2]浙江养生堂天然药物研究所,浙江杭州310007 [3]浙江中医学院,浙江杭州310053 [4]辽宁大学环境与生命科学学院,辽宁沈阳110036

出　　处：《中国中医基础医学杂志》2001年第2期22-24,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金!资助课题 (No.3980 0 192 )

摘　　要：目的 :观察自制的养阴益气活血冲剂对沙土鼠脑缺血时儿茶酚胺水平的影响。方法 :沙土鼠灌服不同剂量的养阴益气活血冲剂 ,在水合氯醛麻醉下 ,结扎双侧颈总动脉 30 min造成脑缺血 ,取血后用高效液相色谱——电化学联合检测法测定各组动物血浆儿茶酚胺水平。结果 :缺血沙土鼠血浆中去甲肾上腺素浓度显著增加 (P<0 .0 1 ) ,给予中药后可拮抗去甲肾上腺素的升高 ,与脑缺血组比较差异显著 (P<0 .0 1 )。结论 :沙土鼠结扎双侧颈总动脉可致血中去甲肾上腺素增加 ,自制的养阴益气活血冲剂可阻止血浆去甲肾上腺素升高。Objective: To investigate the effect of home made “ YangYinYiQiHuoXue Granules” on catecholamine levels in plasma of jird during brain ischemia. Methods: Experiments were performed on 60g～80g jird under chloral hydrate anesthesia. Brain ischemia induced by bilateral common carotid ligation for 30 min. A procedure with minimal plasma sample pretreatment, based on reversed phase, ion pair HPLC combined with electrochemical detection has been developed for the determination of the norepinephrine in plasma samples which divided into control, ischemia and fed on “ YangYinYiQiHuoXue Granules” priors to ligation. Results: norepinephrine concentration increased markedly ( P< 0 05) in brain ishemia for 30min. While the ischemia animal fed on “ YangYinYiQiHuoXue Granules ” priors to ischemia 30min, comparing ischemia group, norepinephrine concentration decreased markedly ( P< 0 05). Conclusion: Ischemia induced by bilateral common carotid ligation leads to increment of plasma norepinephrine and “ YangYinYiQiHuoXue Granules” can prevent this increasing.

关 键 词：养阴益气活血冲剂 脑缺血 去甲肾上腺素 中医药疗法 

分 类 号：R277.73[医药卫生—中医学] R289.1