鞘内注射神经节苷酯GM-1对大鼠脊髓损伤后慢性中枢性痛的影响  

Effect of intrathecal ganglioside GM-1 on chronic central pain following spinal cord injury in rats

作　　者：谢永刚[1] 李爱芝[1] 姜秀良[1] 马加海[1] 

机构地区：[1]青岛大学医学院附属毓璜顶医院麻醉科, 烟台市264000

出　　处：《中华麻醉学杂志》2014年第2期183-185,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价鞘内注射神经节苷酯GM-1对大鼠脊髓损伤后慢性中枢性痛的影响.方法 雄性成年SD大鼠,体重220 ～ 250 g,进行鞘内置管取鞘内置管成功的大鼠32只,采用随机数字表法,将大鼠分成4组（n=8）：对照组（C组）、慢性中枢性痛组（CCP组）、生理盐水组（N组）和神经节苷酯GM-1组（GM 组）.CCP 组、N组和GM组采用改良Allen法建立大鼠脊髓损伤后慢性中枢性痛模型.造模成功后第14天,GM组鞘内注射神经节苷酯GM-1生理盐水稀释液20 mg/kg,1次/d1,连续5d;N组鞘内注射生理盐水10 μl. 于给药结束后4、8、16、24和48 h时测定机械痛阈和热痛阈.于给药结束后7d处死大鼠,取L41节段脊髓组织,采用免疫组化法测定含1亚基的NMDA受体（NR1）的表达水平.结果 与C组比较,CCP组和N组机械痛阈和热痛阈降低,脊髓NR1表达上调（P＜0.01）,GM组机械痛阈、热痛阈和脊髓NR1表达差异无统计学意义（P＞0.05）;与CCP组比较,N组机械痛阈、热痛阈和脊髓NR1表达差异无统计学意义（P＞0.05）,GM组机械痛阈和热痛阈升高,脊髓NR1表达下调（P＜0.05）.结论 鞘内注射神经节苷酯GM-1可减轻大鼠脊髓损伤后慢性中枢性痛,其机制与抑制脊髓NR1的表达有关.Objective To evaluate the effect of intrathecal ganglioside GM-1 on chronic central pain （CCP） following spinal cord injury in rats.Methods Thirty-two adult male Sprague-Dawley rats,weighing 220-250 g,in which intrathecal catheters were successfully implanted,were randomly divided into 4 groups （n =8 each） using a random number table：control group （group C）; group CCP; normal saline group （group N） and ganglioside GM-1 group （group GM）.CCP was induced according to modified Allen method in CCP,N and GM groups.In group GM,ganglioside GM-1 20 mg/kg was injected intrathecally once a day,for 5 consecutive days,starting from 14th day after CCP,while the equal volume of nomal saline 10 μl was injected intrathecally in group N.The mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 4,8,16,24 and 48 h after the end of administration.The rats were then sacrificedat at 7 d after the end of adminmistration and L1 segment of the spinal cord was removed for determination of the expression of NMDA receptor 1 （NR1） by immuno-histochemistry.Results Compared with group C,MWT and TWL were significantly decreased,and NR1 expression was up-regulated in CCP and N groups （P 〈 0.01）,and no significant changes were found in MWT,TWL and NR1 expression in group GM （P 〉 0.05）.Compared with group CCP,no significant changes were found in MWT,TWL and NR1 expression in group N （P 〉 0.05）,and MWT and TWL were significantly increased,and NR1 expression was down-regulated in group GM （P 〈 0.05）.Conclusion Ganglioside GM-1 can alleviate CCP following spinal cord injury in rats and inhibition of expression of NR1 in the spinal cord may be involved in the mechanism.

关 键 词：神经节苷酯类 脊髓损伤 疼痛 注射 脊髓 

分 类 号：R651.2[医药卫生—外科学] R96[医药卫生—临床医学]