异氟醚后处理对缺血缺氧性脑损伤新生大鼠脑组织血管内皮生长因子表达的影响  

Effect of isoflurane postconditioning on expression of vascular endothelial growth factor in neonatal rats with hypoxic-ischemic brain injury

作　　者：池晓颖[1] 赵晓春[1] 吴秀英[1] 郭瑶[1] 张立民[1] 马铃[1] 赵平[1] 陈卫民[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科, 沈阳市110004

出　　处：《中华麻醉学杂志》2014年第2期203-206,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金

摘　　要：目的 探讨异氟醚后处理对缺血缺氧性脑损伤新生大鼠脑组织血管内皮生长因子（VEGF）表达的影响.方法 出生后7d的SD大鼠156只,雌雄不拘,采用随机数字表法,将其分为3组（n=52）：假手术组（S组）、缺血缺氧性脑损伤组（HIBI组）和异氟醚后处理组（Ⅰ组）.采用结扎左颈总动脉＋低氧（吸入8％湿化氧气-92％氮气）2 h的方法建立缺血缺氧性脑损伤模型.Ⅰ组于低氧2h时开始吸入1％异氟醚1h,HIBI组吸入30％氧气-70％氮气1h.S组仅做颈动脉分离,不制备模型,其余同HIBI组.于模型制备结束后1、2、3和7 d（T1-4）时,取受损侧脑组织,观察病理学结果,计数海马和皮层死亡神经元,分别采用免疫组化法和Western blot法测定VEGF的表达水平.于T2时测定脑梗死体积百分比.结果 仅S组未见脑梗死发生;与S组比较,HIBI组和Ⅰ组T1-4时受损侧海马和皮层死亡神经元计数升高,VEGF表达上调（P＜0.05）;与HIBI比较,Ⅰ组脑梗死体积百分比降低,T1.2时受损侧海马和皮层死亡神经元计数降低,VEGF表达上调（P＜0.05）,S组各时点受损侧海马和皮层死亡神经元计数和VEGF表达比较差异无统计学意义（P＞0.05）,HIBI组T3时、Ⅰ组T2时VEGF表达水平达高峰（P＜0.05）.Ⅰ组受损侧脑组织病理学损伤较HIBI组减轻.结论 异氟醚后处理可能通过上调脑组织VEGF表达,减轻新生大鼠缺血缺氧性脑损伤.Objective To investigate the effect of isoflurane postconditioning on the expression of vascular endothelial growth factor （VEGF） in neonatal rats with hypoxic-ischemic brain injury （HIBI）.Methods One hundred and fifty-six 7-day-old Sprague-Dawley rats were randomly divided into 3 groups （n =52 each） using a random number table：sham operation group （S group）,HIBI group,and isoflurane post-conditioning group （Ⅰ group）.Brain ischemia was induced by ligation of the left common carotid artery followed by inhalation of 8% O2 ＋ 92% N2 for 2 h at 37 ℃.The rats were exposed to 1% isoflurane for 1 h （group Ⅰ） or to 30% oxygen-70% nitrogen for 1 h （HIBI group） starting from 2 h of hypoxia.The left common carotid artery was only separated and then the rats were exposed to 30% oxygen and 70% nitrogen for 1 h in S group.The rats were sacrificed at 1,2,3 and 7 days after HIBI （T1-4）,and their brains on the damaged side were harvested for microscopic examination and for determination of the dead neuron counts in the hippocampi and cortex and expression of VEGF （by immunohistochemistry and Western blot）.The cerebral infarct size was detected at T2.Results No cerebral infarct was observed in S group.Compared with S group,the cerebral infarct size and counts of dead neurons in the hippocampi and cortex on the damaged side at T1-4 were significantly increased,and the expression of VEGF was up-regulated in H IBI and Ⅰ groups （P 〈 0.05）.Compared with HIBI group,the cerebral infarct size and counts of dead neurons in the hippocampi and cortex on the damaged side at T1,2 were significantly decreased,and the expression of VEGF was up-regulated in Ⅰ group （P 〈 0.05）.There was no significant difference in the counts of dead neurons in the hippocampi and cortex on the damaged side and expression of VEGF between the time points in S group （P 〉 0.05）.The expression of VEGF peaked at T3 in HIBI group and T2 in Ⅰ group （P 〈 0.05）.The pathological changes of brain tissu

关 键 词：异氟醚 缺血后处理 缺氧缺血 脑 婴儿 新生 血管内皮生长因子 

分 类 号：R614[医药卫生—麻醉学]