血红素氧合酶-1在远隔肢体缺血预处理减轻兔肺缺血再灌注损伤中的作用  

Role of haeme oxygenase-1 in remote limb ischemic preconditioning-induced attenuation of lung ischemia-reperfusion injury in rabbits

作　　者：刘东[1] 汪惠文[1] 耿智隆[1] 陈烨[1] 殷裕雄[1] 

机构地区：[1]兰州军区兰州总医院麻醉科,730050

出　　处：《中华麻醉学杂志》2014年第2期219-222,共4页Chinese Journal of Anesthesiology

基　　金：兰州军区科研项目（LHX-2008022）

摘　　要：目的 评价血红素氧合酶-1（HO-1）在远隔肢体缺血预处理减轻兔肺缺血再灌注损伤中的作用.方法 日本大耳白兔24只,4～5月龄,体重2.0～2.5 kg,采用随机数字表法,将其分为4组（n=6）：假手术组（S组）、缺血再灌注组（I/R组）、远隔肢体缺血预处理组（RLIP组）和HO-1抑制剂锌原卟啉＋远隔肢体缺血预处理组（ZnPP＋ RLIP组）.采用结扎左肺门60 min、再灌注180 min的方法制备肺缺血再灌注损伤模型;结扎肺门前行后肢缺血预处理：双后肢缺血10 min,再灌注10 min,重复3次;ZnPP＋ RLIP组后肢缺血预处理前10 min静脉注射锌原卟啉10μrmol/kg.于再灌注结束时,采集动脉血样,进行血气分析;取肺组织,光镜下观察病理学结果,进行肺损伤评分（LIS评分）;测定肺组织湿/干重（W/D）比值、髓过氧化物酶（MPO）活性、丙二醛（MDA）含量、HO-1表达及其活性.结果 与S组比较,I/R组PaO2降低,肺组织LIS评分、W/D比值、MPO活性、MDA含量、HO-1表达及其活性升高（P＜0.01）;与I/R组比较,RLIP组PaO2、肺组织HO-1表达及其活性升高,肺组织LIS评分、W/D比值、MPO活性和MDA含量降低（P＜0.01）;与RLIP组比较,ZnPP＋ RLIP组PaO2、肺组织HO-1表达及其活性降低,肺组织LIS评分、W/D、MPO活性和MDA含量升高（P＜0.01）.结论 远隔肢体缺血预处理通过上调HO-1表达及增强其活性,减轻兔肺缺血再灌注损伤.Objective To evaluate the role of haeme oxygenase-1 （HO-1） in remote limb ischemic preconditioning （RLIP）-induced attenuation of lung ischemia-reperfusion （I/R） injury in rabbits.Methods Twenty-four Japanese White Rabbits,aged 4-5 months,weighing 2.0-2.5 kg,were randomly divided into 4 groups （n =6 each） using a random number table：sham operation group （S group）,I/R group,RLIP group and zinc protoporphyrin （ZnPP,an inhibitor of HO-1） plus RLIP group （ZnPP ＋ RLIP group）.Lung I/R was produced by 60 min occlusion of the left lung hilum followed by 180 min of reperfusion in I/R,RLIP and ZnPP ＋ RLIP groups.RLIP and ZnPP ＋ RLIP groups received 3 cycles of 10 min ischemia followed by 10 min reperfusion in the bilateral hind limbs immediately before occlusion of the left lung hilum.In ZnPP ＋ RLIP group,ZnPP 10 μmol/kg was injected intravenously 10 min prior to hind limb ischemia and the rest of the procedures were similar to those previously described in RLIP group.At the end of reperfusion,arterial blood samples were collected for blood gas analysis.The animals were then sacrificed and pulmonary specimens were obtained for microscopic examination of the pathological changes which were scored （lung injury score,LIS） and for determination of wet/dry lung weight ratio （W/D ratio）,myleoperoxidase （MPO） activity,malondialdehyde （MDA） content and expression and activity of HO-1 in the lung tissues.Results Compared with group S,PaO2 was significantly decreased,and LIS,W/D ratio,MPO activity,MDA content,and HO-1 expression and activity were increased in I/R group （P 〈 0.01）.Compared with I/R group,PaO2 and HO-1 expression and activity were significantly increased,and LIS,W/D ratio,MPO activity and MDA content were decreased in RLIP group （P 〈 0.01）.Compared with RLIP group,PaO2 and HO-1 expression and activity were significantly decreased,and LIS,W/D ratio,MPO activity and MDA content were increased in ZnPP ＋ RLIP group （P 〈 0.01）.Conclusion RLIP up-regu

关 键 词：血红素加氧酶-1 缺血预处理 四肢 再灌注损伤 肺 

分 类 号：R614[医药卫生—麻醉学]