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作 者:商慧文 陈江华 张艳玲[1] 戴华鑫[1] 张仕祥[1]
机构地区:[1]中国烟草总公司郑州烟草研究院 [2]中国烟叶公司
出 处:《烟草科技》2014年第4期88-92,共5页Tobacco Science & Technology
基 金:郑州烟草研究院科技项目"典型烟区土壤环境质量评价及监测监控研究"(112008CZ0310)
摘 要:采用土壤盆栽和水培试验,以不同Cd积累模式的两种基因型烟草[普通烟草品种K326和黄花烟草(N.rustica)]为试材,研究了不同镉浓度处理下烟草根系分泌的低分子量有机酸总量及各组分含量差异。结果表明:①土壤盆栽试验中,1 mg/kg镉处理可促进烟苗生长,10 mg/kg镉处理严重抑制烟苗生长。与K326相比,N.rustica具有更强的耐镉性。②土壤盆栽和水培试验中,低浓度镉处理可促进低分子量有机酸的分泌,高浓度则抑制其分泌。N.rustica低分子量有机酸的分泌总量大于K326。镉处理下,黄花烟草分泌较多的低分子量有机酸可能是其耐镉的主要生理机制之一。③土壤盆栽和水培试验中,N.rustica根系分泌的草酸、酒石酸、甲酸和乳酸含量均高于K326,但这4种有机酸占有机酸总量的比例两烟草基因型间差异均不显著。两基因型烟草的耐镉性差异可能与这4种低分子量有机酸的分泌量有关,而与各有机酸的组成比例无关。Soil pot and water culture experiments of two tobacco genotypes of different Cd accumulating patter, N. tabacurn (K326) and N. rustica, were carried out to study their difference in total and individual contents of low molecular weight organic acids (LMWOA) in the exudates of root system. The results showed that: l) In soil pot experiment, tobacco seedling growth was promoted by Cd treatment at the rate of 1 mg/kg, while it was seriously inhibited at Cd rate of 10 mg/kg. N. rustica was more tolerant to Cd than was K326. 2) In soil pot and water culture experiments, LMWOAs secretion was promoted at low Cd level, it was inhibited at high Cd level. Total amount of LMWOAs exudates by IV. rustica was higher than that by K326, which indicated that higher LMWOAs exudation might be one of the major factors leading to higher tolerance of N. rustica to Cd. 3) The contents of oxalic acid, tartaric acid, methanoic acid and lactic acid were higher in N. rustica than in K326, however, the proportions of the four organic acids in total organic acids were not significantly different between the two genotypes; the different tolerance to Cd of the two genotypes probably related to the amounts of the four organic acids, rather than the proportion of each organic acid.
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