泡桐属植物遗传多样性的FISH-AFLP分析  

FISH-AFLP analysis of genetic dversity of genus Paulownia plants

作　　者：李翰书 卢妍妍[1] 李海英[1,2] 茹广欣[1] 杨阳[1] 候婷[1] 杨永红[1] 

机构地区：[1]河南农业大学林学院,河南郑州450002 [2]华北水利水电学院,河南郑州450002

出　　处：《河南农业大学学报》2014年第2期140-144,共5页Journal of Henan Agricultural University

基　　金：国家科技支撑计划专题项目(2013BAD01B06);郑州市科技领军人才计划项目(10LRC177)

摘　　要：运用FISH—AFLP技术对泡桐属14个种及变种进行了基因组DNA分子水平上的检测．结果表明，从64对AFLP引物组合中筛选出9对，共获得1171条清晰可辨的条带，其中多态性条带1158条，多态性带百分率达98．89％，14份供试材料的遗传相似系数为0．6034—0．7402，平均相似系数为O．6718，反映出泡桐属不同种质间差异明显，具有丰富的多态性．本研究运用NTSYS2．1版软件对样品进行UPGMA聚类，分析了泡桐属植物14个种的遗传多样性和亲缘关系，并对泡桐属部分植物的分类问题进行讨论．The FISH-AFLP analysis was conducted in order to investigate the sibship among the 14 ge- nus Paulownia plants. A total of 1 171 DNA fragments were amplified using 9 out of 64 AFLP primers with unambiguous unique polymorphic bands,among which 1 158 DNA bands were polymorphic and the average percentage of polymorphic bands was 98.89%. The genetic similarity indexes among the 14 genus Paulownia plants ranged from 0. 603 4 to 0. 740 2 with an average of 0. 671 8 ,indicating signifi- cant genetic differentiation among Paulownia accessions tested. NTSYS 2.1 version of the software was used to analyze the genetic diversity and genetic relationship between the 14 genus Paulownia plants, and the taxonomy of some Paulownia species was discussed.

关 键 词：泡桐属 遗传多样 FISH—AFLP 聚类分析 亲缘关系 

分 类 号：S792[农业科学—林木遗传育种]