分子杂交法研究肝炎病人血清和肝组织中输血传播病毒  

Study on transfusion transmitted virus in serum and liver tissue by molecular hybridization

作　　者：阎惠平[1] 周育森[2] 郎振为[1] 黄德庄[1] 金荣华[1] 罗朝霞[1] 贺立香[1] 王海涛[2] 

机构地区：[1]北京佑安医院,北京市肝炎研究所 [2]军事医学科学院五所

出　　处：《中华微生物学和免疫学杂志》2001年第1期18-20,共3页Chinese Journal of Microbiology and Immunology

基　　金：北京市自然科学基金会资助项目 (7992 0 2 3)

摘　　要：目的　对各型肝炎病人血清和肝组织中输血传播病毒 (TTV)核酸检测分析 ,探讨病毒的致病性。方法　以地高辛为标记物制备TTVDNA探针 ,斑点杂交法、原位杂交法分别检测血清中及肝组织中TTVDNA。结果　检测 10 3例血清 ,TTV总阳性率为 2 5 2 4% (2 6 /10 3) ;甲～戊型肝炎组检出率 2 1 81% (12 /5 5 )、非甲～非庚型肝炎患者检出率 47 37% (9/19) ,显著高于正常对照组 15 % (3/2 0 )。临床可见TTV的单独感染和重叠感染 ;出现急性、慢性甚至重度肝损伤。 12例肝组织可见TTV阳性 ,阳性颗粒主要见于肝细胞核内。结论　从血清和肝组织证实了TTV的存在 。Objective To detect and analysis a novel virus transfusion transmitted virus (TTV) and its infection in serum and liver tissue of patients with hepatitis. Methods TTV DNA probe was labeled with digoxigenin. TTV DNA, in serum was tested by dot blot hybridization and in liver by in situ hybridization. Results Of 103 tested sera the prevalence of TTV was 25.24% (26/103). In the group of A E types hepatitis, the positive percentage of TTV was 21.82% (12/55), in the patients with non A non G types hepatitis (47.37%, 9/19), it was significantly higher than it in control group (15%, 3/20). Both TTV alone infection or superinfection were seen in clinic and acute, chronic type even severe liver injures were present. Of 12 liver samples, TTV DNA was observed in nuclei of hepatocytes. Conclusion TTV was demonstrated in serum and liver of patients with hepatitis; These evidence showed that TTV is probably a novel hepatitis virus.

关 键 词：肝炎病毒 原位杂交 肝组织 TTV 核酸探针 

分 类 号：R346[医药卫生—基础医学]